Objective To investigate the effects of tumor necrosis factor α-induced protein 8-like 1 (TIPE1) on the proliferation,migration,invasion,and epithelial-mesenchymal transition (EMT) of colorectal cancer (CRC) cells and to analyze its potential biolog-ical mechanisms.Methods RT-qPCR was used to measure TIPE1 mRNA levels in CRC tissues and cell lines.After over-expressing TIPE1 using lentiviral transduction,cell viability was assessed using the CCK-8 assay,while wound healing and Transwell assays were performed to evaluate cell migration and invasion.Western blotting was employed to detect the expression of proteins related to TGF-β/Smad/EMT signaling in the cells.Subcutaneous xenografts were established by injecting SW480 cells over-expressing TIPE1 into nude mice.Animals were sacrificed after 21 days,and tumor volumes were measured.Immunohistochemical staining was used to analyze the expression of TGF-β/Smad/EMT signaling-related proteins in tumor tissues.Results TIPE1 mRNA expression was down-regulated in CRC tissues and cells.Over-expression of TIPE1 in CRC cells reduced cell viability,migration,and invasion.Western blot results showed that over-expression of TIPE1 increased E-cadherin levels while decreasing vimentin,TGF-β1,and p-Smad2 levels in CRC cells.In the nude mouse model,the TIPE1 overexpression group exhibited slower tumor growth and smaller tumor volumes.Immunohistochemical staining showed upregulated E-cadherin and downregulated vimentin,TGF-β1,and p-Smad2 in tumors from the TIPE1 over-expression group.Conclusion Over-expression of TIPE1 inhibits CRC cell proliferation,migration,invasion,and EMT,potentially through suppression of TGF-β/Smad signaling activity.
关键词
结直肠癌/肿瘤坏死因子α诱导蛋白8样分子1/上皮-间质转化/迁移/侵袭/TGF-β/Smad信号
Key words
Colorectal cancer/tumor necrosis factor α-induced protein 8-like 1/epithelial-mesenchymal transition/migration/invasion/TGF-β/Smad signaling
维普
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