Objective To investigate the effect and mechanism of scutellarin (SC) on cognitive impairment in mice with Alzhei-mer's disease (AD).Methods Twenty APP/PS1 transgenic mice were divided into AD model group and SC intervention group,with 10 mice in each group.Another 10 wild-type mice were selected as the control group.SC intervention group was given 50 mg/kg SC orally once a day for 2 months.Morris water maze test was used to detect the cognitive behavior of mice in each group.Nissl staining and Golgi fluorescent labeling staining were used to detect the synaptic plasticity of hippocampal neurons.Western blot was used to detect the expression of postsynaptic density 95 (PSD95) and synaptophysin (SYN) protein.Electrophysiology was used to measure the long-term potentiation effect (LTP) in isolated hippocampal slices.Results Compared to the control group,the AD model group exhibited increased escape latency and neuron loss,decreased residence time and crossing times in the original platform of mice,as well as weakened hippocampal synaptic plasticity and LTP.In comparison to the AD group,the SC intervention group showed reduced escape latency and neuron loss,increased residence time and crossing times in the original platform,along with enhanced hippocampal synaptic plasticity and LTP.Conclusion SC can alleviate cognitive impairment in APP/PS1 transgenic mice,and its effects may be related to reducing hippocampal neuron loss,synaptic damage and enhancing LTP.
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