Objective To explore methods to improve issues such as deep background staining and silver particle deposition in reticular fiber staining.Methods This study optimized the commonly used drop staining method to develop a simulated immersion staining method.The staining results obtained by the two methods were compared and analyzed.Results The simulated immersion staining method for reticular fibers was superior to the drop staining method in terms of reducing staining background,improving clarity,and decreasing silver particle deposition.Conclusion The simulated immersion staining method can significantly improve the quality of reticular fiber staining.
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