Preparation and Application of Manganese-loaded Activated Carbon-Cellulose Composite Adsorbent for Enhancing Laccase Enzyme Activity
A novel manganese-loaded activated carbon-cellulose composite adsorbent that could immobilize laccase and increase the enzyme activity was prepared by solvent regeneration method,using waste manganese-loaded activated carbon and cellulose,and further prepared the laccase-carrier material after immobilizing laccase.The results showed that the content of Mn2+had a certain effect on the enzyme activity of the laccase-carrier material,and the enzyme activity of the laccase-carrier material was the highest when the content of Mn2+in the composite adsorbent was 1.5 mg/g.The optimum process conditions for the immobilization of laccase were the immobilization temperature of 45℃,the pH value of 4,and the initial enzyme concentration of 6.5 g/L.The immobilization ratio of the laccase-carrier material was the highest to 48.24%under the optimum conditions of immobilization;the thermal and pH stabilities of the laccase-carrier material were significantly en-hanced;the degradation rate of bisphenol A using the laccase-carrier material reached 68%,and the relative degradation rate of bisphenol A was still up to 51%after 5 times of reuse.
waste manganese-loaded activated carboncellulose-based adsorbentimmobilized laccasebisphenol A degradation
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