Study on the Starch Enzymolysis Performance of α-Amylase and Its Complex Enzyme System
This study investigated the enzymatic performance of single α-amylase and binary,ternary,and quaternary complex enzyme systems consisting of α-amylase with β-amylase,pullulanase and glucoamylase for three commonly used modified starches(oxidized starch,enzymati-cally hydrolyzed starch,and cationic starch).The effect of different α-amylase dosages on starch enzymatic rate and DE value was explored.The results indicated that when the starch enzymatic rate reached 90%in single α-amylase system,the required amount of α-amylase for cat-ionic starch enzymolysis was about 4 times than that of the other two kinds of starches.The enzymatic rate of enzymatically hydrolyzed starch could be increased by up to 37.4%when citric acid was added to inhibit zinc ions.In the binary complex enzyme system,the enzymatic rate of cationic starch by α-amylase/β-amylase was 5.3%and 8.7%higher than that by α-amylase/glucoamylase and α-amylase/pullulanase,re-spectively.In the ternary complex enzyme system,the enzymatic rate of cationic starch by α-amylase/glucoamylase/β-amylase was 1.2%higher than that by α-amylase/glucoamylase/pullulanase.In the application of α-amylase/glucoamylase/β-amylase/pullulanase tetrameric com-plex enzyme system to the enzymatic reactions of cationic starch,enzymatically hydrolyzed starch and oxidized starch,when the enzymatic rate of starch reached 80%,the required amount of α-amylase was 56%,38%and 17%of that by single α-amylase system,respectively.
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