摘要
目的 探讨Six1和Six4在食管鳞状细胞癌(简称鳞癌)组织中的表达及其与临床病理特征及预后的关系.方法 应用组织芯片技术、免疫组织化学EnVision法检测Six1和Six4在292例食管鳞癌组织及其相应的癌旁正常食管上皮组织中的表达水平,分析两者表达与临床病理特征及预后之间的关系.结果 在292例食管鳞癌患者中,Six1、Six4蛋白在癌组织的阳性率分别为72.9％(213/292)和56.2％(164/292),明显高于癌旁正常组织的阳性率,分别为33.2％ (97/292)和32.5％(95/292),差异具有统计学意义(P＜0.05).x2检验结果显示Six1蛋白表达与肿瘤大小、肿瘤浸润深度及生存状态相关,其在死亡组患者的阳性率82.3％ (130/158),明显高于生存组阳性率61.9％(83/134,P＜0.05).Six4蛋白表达与肿瘤分化程度及肿瘤浸润深度相关,肿瘤分化程度越高、浸润深度越深其阳性率越高.单因素Log-rank分析显示性别、TNM分期、Six1蛋白表达与食管鳞癌患者的总生存率相关(P＜0.05).Six1蛋白表达阴性组5年生存率51.9％ (41/79)高于阳性组的43.7％(93/213),差异具有统计学意义(x2 =4.079,P =0.043),其他临床参数与预后均未显示统计学意义(P>0.05).多因素Cox比例风险模型分析显示,TNM分期、Six1蛋白表达水平是影响食管癌术后患者预后的独立因素(P＜0.05).结论 Six1和Six4蛋白在食管鳞癌组织中均高表达,其表达水平与食管鳞癌发生发展及预后密切相关,Six1蛋白高表达是食管鳞癌患者预后不良的因素,可作为临床预测食管鳞癌患者预后的一个重要指标.
Abstract
Objective Six1 and Six4 are expressed in several tumors,and associated with tumor progress and poor prognosis.The aim of this study was to investigate the expression of Six1 and Six4 in esophageal squamous cell carcinoma (ESCC),and to evaluate their correlation with the clinicopathological factors and prognosis.Methods Tissue microarray technology and immunohistochemical method (EnVision) were used to detect the expression of Six1 and Six4 in the tumor tissues and corresponding adjacent normal epithelium of esophagus from 292 ESCC patients.Results Among the 292 ESCC patients,the positive rates of Six1 and Six4 protein expression in tumor tissues were 72.9％ (213/292) and 56.2％ (164/292),respectively,significantly higher than the expression rate of 33.2％ (97/292) and 32.5％ (95/292) in adjacent normal epithelium of esophagus (P ＜ 0.05).Chi square test showed that the expression of Six1 protein was related to tumor size,depth of tumor invasion and patient survival status;higher Six4 protein expression level was related to poor differentiation and increased depth of invasion.Single factor Log-rank analysis revealed that gender,TNM stage,Six1 protein expression level were related to the overall survival of ESCC patients (P ＜ 0.05),while the five-year survival rate was significantly higher in the Six1-negative group than the Six1-positive group [51.9％ (41/79) vs.43.7％ (93/213)].Multi-factor Cox proportional risk model analysis showed that TNM stage and positive expression of Six1 were independent prognostic factors for ESCC patients (P ＜ 0.05).Conclusions Six1 and Six4 are highly expressed in ESCC.Their expression levels are closely related to the progress and prognosis of ESCC.Over-expression of Six1 is related to poor prognosis in ESCC patients.Thus,Six1 could be used as an important prognostic indicator for ESCC patients.
基金项目
国家临床重点专科项目()
卫生部部属医院临床学科重点项目()
上海市科学技术委员会专项基金重点项目(10JC1419400)
NETL
NSTL
维普
万方数据