Clinicopathological features of gastric alpha-fetoprotein-producing adenocarcinoma with SWI/SNF complex deletion
Objective To investigate the clinicopathological features and treatment of gastric alpha-fetoprotein(AFP)-producing adenocarcinoma with SWI/SNF complex deletion.Methods Four cases of gastric AFP-producing adenocarcinoma with SWI/SNF complex deletion diagnosed in Zhongshan Hospital of Fudan University from January 2021 to December 2022 were collected,and their histomorphological characteristics,immunohistochemical(IHC),in situ hybridization of Epstein-Barr virus-encoded RNA(EBER),next-generation sequencing results,clinicopathological features and treatment were summarized,and literature review was conducted.Results Among the 4 patients,there were three males and one female.They presented with abdominal pain,belching and melena.Serum AFP was significantly elevated in three patients,and endoscopy showed ulcerative lesions.Microscopically,the tumor cells showed mainly diffuse flaky or nest-like growth and typical characteristics of hepatoid adenocarcinoma.In two cases there were adenoid growth,and the tumor cells in these areas possessed clear cytoplasm,suggesting enteroblastic differentiation.The tumor cell nuclei were pleomorphic with large nucleoli and brisk mitoses.The IHC results showed that the tumor cells expressed AFP,GPC3 and SALL4,and there was retained expression of broad-spectrum keratin(CKpan)and E-cadherin.IHC detection of SWI/SNF complex subunits,namely INI1(SMARCB1),BRG1(SMARCA4),BRM(SMARCA2),ARID1A protein was performed.In all four cases the hepatoid adenocarcinoma region and enteroblastic differentiation region showed SMARCA2 deletion,and one case with enteroblastic differentiation also showed ARID1A deletion.SMARCB1 and SMARCA4 deletions were not seen.All the four cases were diffusely positive for p53 protein,and the Ki-67 proliferation index was 80％-90％.There were no mismatch repair deletion detected;one cases showed HER2 was strongly positive(3+),and EBER was negative.None of the four cases had mutations in the SWI/SNF complex-related subunits detected by next-generation sequencing.Among the four patients,two underwent palliative surgery due to distant metastasis at the time of surgery,two underwent radical resection.Postoperative adjuvant chemotherapy was given to three patients.Conclusions AFP-producing adenocarcinoma is a rare subtype of gastric cancer,which can be combined with SWI/SNF complex deletion,and the pathomorphological manifestations are different from the classical SWI/SNF complex deletion of undifferentiated carcinoma with rhabdoid phenotype.
NETL
NSTL
万方数据
维普
