首页|Using a cDNA microarray to study cellular gene expression altered by Mycobacterium tuberculosis

Using a cDNA microarray to study cellular gene expression altered by Mycobacterium tuberculosis

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Objective To examine the global effects of Mycobacterium tuberculosis (M.tuberculosis) infection on macrophages. Methods The gene expression profiling of macrophage U937, in response to infection with M.tuberculosis H37Ra, was monitored using a high-density cDNA microarray. Results M.tuberculosis infection caused 463 differentially expressed genes, of which 366 genes are known genes registered in the Gene Bank. These genes function in various cellular processes including intracellular signalling, cytoskeletal rearrangement, apoptosis, transcriptional regulation, cell surface receptors, cell-mediated immunity as well as a variety of cellular metabolic pathways, and may play key roles in M.tuberculosis infection and intracellular survival. Conclusions M.tuberculosis infection alters the expression of host-cell genes, and these genes will provide a foundation for understanding the infection process of M.tuberculosis. The cDNA microarray is a powerful tool for studying pathogen-host cell interaction.

macrophageMycobacterium tuberculosisgene expression

徐永忠、谢建平、李瑶、乐军、陈建平、淳于利娟、王洪海

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State Key Laboratory of Genetic Engineering, Institute of Genetics , Fudan University, Shanghai 200433, China

Department of Microbiology, Fudan University, Shanghai 200433, China

国家自然科学基金国家重点基础研究发展计划(973计划)

30100007G199054104

2003

中华医学杂志(英文版)
中华医学会

中华医学杂志(英文版)

SCI
影响因子:0.838
ISSN:0366-6999
年,卷(期):2003.116(7)
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