首页|A recommended technique of renal vein anastomosis in rat kidney transplantation for trainee

A recommended technique of renal vein anastomosis in rat kidney transplantation for trainee

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Background Various rat kidney transplantation models have been introduced over the decades and the study on the models seems to lack novelty and necessity.However,vascular anastomosis,especially renal vein,is still very difficult for trainees.The aim of this study was to provide the modified renal venous anastomosis of rat kidney transplantation to substitute the currant method for trainees.Methods Male Wistar rats were used as donors and recipients,respectively.Left orthotopic transplantation was performed with a modified technique of renal vein anastomosis,combining the end-to-end sutures with epidural catheter.Meanwhile,the survival rate,warm ischemia time,renal venous anastomosis time,and complications were recorded to evaluate the merits of the modified technique compared with the current recommended technique of rat renal vein.Two trainees took part in the learning of the models in two methods for performing 30 operations,respectively.Results The difference in warm ischemia time (from (57.25±7.30) minutes in the first 10 operations to (30.05±1.85)minutes in the third 10 operations) and renal vein anastomosis time (from (32.80±3.80) minutes in the first 10 operations to (19.30±0.98) minutes in the third 10 operations) was significantly short (P<0.01) and the survival rate was statistically high (from (25±7)% in the first 10 operations to 70% in the third 10 operations) in equal number of operations (P<0.01) by comparing with the current recommended method ((47.60±7.19) minutes to (22.8±1.85) minutes,(22.40±3.10) minutes to (9.95±1.50) minutes,45%±7% to 80%±0,respectively).The intraoperative complications and postoperative complications of renal venous anastomosis were also significantly decreased (P<0.01).Conclusions The technique with epidural catheter can shorten the learning curve of the trainee learning rat kidney transplantation.It may replace the currently recommended technique of rat renal vein for trainees.

ratskidney transplantationtrainee

Ye Dongming、Heng Baoli、Lai Caiyong、Guo Zexiong、Su Zexuan

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Graduate School of Southern Medical University, Guangzhou,Guangdong 510515, China

Department of Urology, First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China

Department of Renal Transplantation, First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China

National "863" ProjectScience and Technology Research Projects of Guangzhou

2007AA02200611A72070508

2014

中华医学杂志(英文版)
中华医学会

中华医学杂志(英文版)

CSTPCDCSCDSCI
影响因子:0.838
ISSN:0366-6999
年,卷(期):2014.127(10)
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