首页|Alterations in nasal microbiota of patients with amyotrophic lateral sclerosis

Alterations in nasal microbiota of patients with amyotrophic lateral sclerosis

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  • 国家科技期刊平台
  • NETL
  • NSTL
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Background:Links between alterations in gut microbiota composition and amyotrophic lateral sclerosis(ALS)have previously been reported.This study aimed to examine the microbiota in the nasal cavity of ALS.Methods:Sixty-six ALS patients and 40 healthy caregivers who live in close proximity with patients were enrolled.High throughput metagenomic sequencing of the 16S ribosomal deoxyribonucleic acid(rDNA)gene V3-V4 region of nasal microbiota was used to characterize the alpha and beta diversity and relative abundance of bacterial taxa,predict function,and conduct correlation analysis between specific taxa and clinical features.Results:The nasal microbiome of ALS patients showed lower alpha diversity than that of corresponding healthy family members.Genera Gaiella,Sphingomonas,Polaribacter_1,Lachnospiraceae_NK4A136_group,Klebsiella,and Alistipes were differentially enriched in ALS patients compared to controls.Nasal microbiota composition in ALS patients significantly differed from that in healthy subjects(unweighted UniFrac P=0.001),while Linear discriminant analysis Effect Size(LEfSe)analysis indicated that Bacteroidetes and Firmicutes dominated healthy nasal communities at the phylum level,whereas Actinobacteria was the predominant phylum and Thermoleophilia was the predominant class in ALS patients.Genus Faecalibacterium and Alistipes were positively correlated with ALS functional rating scale revised(ALSFRS-R;rs=0.349,P=0.020 and rs=0.393,P=0.008),while Prevotella-9 and Bacteroides operational taxonomic units(OTUs)were positively associated with lung function(FVC)in ALS patients(rs=0.304,P=0.045,and rs=0.300,P=0.048,respectively).Prevotella-1 was positively correlated with white blood cell counts(WBC,rs=0.347,P=0.021),neutrophil percentage(Neu％,rs=0.428,P=0.004),and neutrophil-to-lymphocyte ratio(NLR,rs=0.411,P=0.006),but negatively correlated with lymphocyte percentage(Lym％,rs=-0.408,P=0.006).In contrast,Streptococcus was negatively associated with Neu％(rs=-0.445,P=0.003)and NLR(rs=-0.436,P=0.003),while positively associated with Lym％(rs=0.437,P=0.003).No significant differences in nasal microbiota richness and evenness were detected among the severe and mild ALS patients.Conclusions:ALS is accompanied by altered nasal microbial community composition and diversity.The findings presented here highlight the need to understand how dysbiosis of nasal microbiota may contribute to the development of ALS.

Amyotrophic lateral sclerosisNasal microbiotaDysbiosisInflammationFaecalibacterium

Kaixiong Liu、Qifu Guo、Ying Ding、Li Luo、Jianchai Huang、Qijie Zhang

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Department of Respiratory and Critical Care Medicine,The First Affiliated Hospital,Fujian Medical University,Fuzhou,Fujian 350005,China

Department of Respiratory and Critical Care Medicine,National Regional Medical Center,Binhai Campus of The First Affiliated Hospital,Fujian Medical University,Fuzhou,Fujian 350212,China

Department of Neurology,Fujian Institute of Neurology,The First Affiliated Hospital,Fujian Medical University,Fuzhou,Fujian 350005,China

Department of Neurology,National Regional Medical Center,Binhai Campus of The First Affiliated Hospital,Fujian Medical University,Fuzhou,Fujian 350212,China

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Natural Science Foundation of Fujian ProvinceNatural Science Foundation of Fujian ProvinceJoint Funds for the Innovation of Science and Technology,Fujian ProvinceFujian Provincial Health Technology Project

2021J012092021J012282021Y91362020GGA053

2024

10.1097/CM9.0000000000002701

中华医学杂志(英文版)
中华医学会

中华医学杂志(英文版)

CSTPCD
影响因子：0.838
ISSN：0366-6999
年,卷(期)：2024.137(2)
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