首页|Severe acute respiratory syndrome coronavirus 2-specific T-cell responses are induced in people living with human immunodeficiency virus after booster vaccination

Severe acute respiratory syndrome coronavirus 2-specific T-cell responses are induced in people living with human immunodeficiency virus after booster vaccination

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
Background:T-cell-mediated immunity is crucial for the effective clearance of viral infection,but the T-cell-mediated immune responses that are induced by booster doses of inactivated severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)vac-cines in people living with human immunodeficiency virus(PLWH)remain unclear.Methods:Forty-five PLWH who had received antiretroviral therapy(ART)for more than two years and 29 healthy controls(HCs)at Beijing Youan Hospital were enrolled to assess the dynamic changes in T-cell responses between the day before the third vaccine dose(week 0)and 4 or 12 weeks(week 4 or week 12)after receiving the third dose of inactivated SARS-CoV-2 vaccine.Flow cytometry,enzyme-linked immunospot(ELISpot),and multiplex cytokines profiling were used to assess T-cell responses at the three timepoints in this study.Results:The results of the ELISpot and activation-induced marker(AIM)assays showed that SARS-CoV-2-specific T-cell responses were increased in both PLWH and HCs after the third dose of the inactivated SARS-CoV-2 vaccine,and a similar magnitude of immune response was induced against the Omicron(B.1.1.529)variant compared to the wild-type strain.In detail,spike-specific T-cell responses(measured by the ELISpot assay for interferon γ[IFN-γ]release)in both PLWH and HCs significantly increased in week 4,and the spike-specific T-cell responses in HCs were significantly stronger than those in PLWH 4 weeks after the third vaccination.In the AIM assay,spike-specific CD4+T-cell responses peaked in both PLWH and HCs in week 12.Additionally,sig-nificantly higher spike-specific CD8+T-cell responses were induced in PLWH than in HCs in week 12.In PLWH,the release of the cytokines interleukin-2(IL-2),tumour necrosis factor-alpha(TNF-a),and IL-22 by peripheral blood mononuclear cells(PBMCs)that were stimulated with spike peptides increased in week 12.In addition,the levels of IL-4 and IL-5 were higher in PLWH than in HCs in week 12.Interestingly,the magnitude of SARS-CoV-2-specific T-cell responses in PLWH was negatively associated with the extent of CD8+T-cell activation and exhaustion.In addition,positive correlations were observed between the magnitude of spike-specific T-cell responses(determined by measuring IFN-γ release by ELISpot)and the amounts of IL-4,IL-5,IL-2 and IL-17F.Conclusions:Our findings suggested that SARS-CoV-2-specific T-cell responses could be enhanced by the booster dose of inactivated COVID-19 vaccines and further illustrate the importance of additional vaccination for PLWH.

HIVPeople living with HIVSARS-CoV-2 vaccineT-cell responsesOmicron variant

Xiuwen Wang、Yongzheng Li、Junyan Jin、Xiaoran Chai、Zhenglai Ma、Junyi Duan、Guanghui Zhang、Tao Huang、Xin Zhang、Tong Zhang、Hao Wu、Yunlong Cao、Bin Su

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Beijing Key Laboratory for HIV/AIDS Research,Sino-French Joint Laboratory for HIV/AIDS Research,Clinical and Research Center for Infectious Diseases,Beijing Youan Hospital,Capital Medical University,Beijing 100069,China

Biomedical Pioneering Innovation Center(BIOPIC),Peking University,Beijing 100069,China

Cancer and Stem Cell Biology Program,Duke-NUS Medical School,Singapore

Changping Laboratory,Beijing 102299,China

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2024

10.1097/CM9.0000000000003176

中华医学杂志(英文版)
中华医学会

中华医学杂志(英文版)

CSTPCD
影响因子：0.838
ISSN：0366-6999
年,卷(期)：2024.137(22)