模拟长期微重力对大鼠耳蜗基底膜p120-catenin表达的影响
Effects of Simulated Microgravity on P120-catenin Expression in Rat Cochlear Basilar Membrane
朱莹蕾 1王刚 2周莹 3王磊 3范博雅 4韩浩伦 3李保卫 3孙喆喆 3吴继华 5吴玮1
作者信息
- 1. 北京大学解放军306医院教学医院(北京 100101);中国人民解放军战略支援部队特色医学中心耳鼻喉科;国家环境保护环境感官应激与健康重点实验室
- 2. 中国人民解放军战略支援部队特色医学中心耳鼻喉科;国家环境保护环境感官应激与健康重点实验室
- 3. 中国人民解放军战略支援部队特色医学中心耳鼻喉科
- 4. 北京大学解放军306医院教学医院(北京 100101);中国人民解放军战略支援部队特色医学中心耳鼻喉科
- 5. 中国人民解放军战略支援部队特色医学中心病理科
- 折叠
摘要
目的 观察模拟长期微重力对大鼠听功能的影响,探讨耳蜗黏附连接蛋白p120-catenin(p120ctn)在耳蜗基底膜表达情况及模拟微重力对其产生的影响.方法 选取健康雄性SD大鼠48只,随机分为模拟微重力组和对照组,每组24只;模拟微重力组和对照组又根据暴露时间分别随机分为1d、1周、8周、12周组,每组6只12耳.在暴露前(B0)、暴露结束后即刻(P0)进行双耳畸变产物耳声发射(distortion product otoacoustic emission,DPOAE)和听性脑干反应(auditory brainstem response,ABR)阈值检测,并于P0测听结束后处死每组大鼠并分离双侧耳蜗,进行免疫组化染色.结果 各组DPOAE引出率比较,差异无统计学意义(P>0.05),ABR阈值随暴露时间的增加而升高,模拟微重力1周组、模拟微重力8周组、模拟微重力12周组P0时ABR阈值较B0时升高,差异有统计学意义(P<0.05).模拟微重力8周组、模拟微重力12周组基底膜上p120ctn阳性表达较对照组显著增高,差异有统计学意义(P<0.05).结论 模拟微重力会导致大鼠听功能损伤,耳蜗基底膜上p120ctn表达随暴露时间的延长而增加,中长期微重力导致听功能下降可能与基底膜p120ctn的表达增加有关.
Abstract
Objective To report effects of simulated microgravity on the expression of cochlear catenin cofactor p120-catenin(p120ctn)in the basilar membrane in rats.Methods Healthy male SD rats(n=48)were randomly divided into a simulated microgravity group and a control group(n=24 in each group),which were further divided subgroups based on exposure time(1 day,1 week,8 weeks and 12 weeks,n=6/12 ears in each subgroup.Prior to and immediately after exposure(B0 and P0),distortion product otoacoustic emissions(DPOAEs)and binaural auditory brainstem re-sponse(ABR)thresholds were obtained.At the conclusion of P0 audiometry,rats were executed and both cochleae iso-lated and stained immunohistochemically.Results No differences in DPOAE detection across the groups(P>0.05).ABR thresholds rose as exposure time increased and higher for 1 week,8 weeks and 12 weeks exposure than at B0(P<0.05).Increased P120-catenin expression was noticed in animals with 8 weeks or 12 weeks microgravity exposure as compared to controls(P<0.05).Conclusion Simulated microgravity negatively affects hearing in rats,with p120ctn ex-pression on cochlear basilar membrane increasing along with exposure time.Auditory function impairment brought on by microgravity may be influenced by p120ctn.
关键词
微重力/听性脑干反应/黏附连接蛋白/p120-cateninKey words
microgravity/auditory brainstem response/adhesion junction protein/p120-catenin引用本文复制引用
基金项目
军队医学科技青年培育计划孵化项目(20QNPY067)
航天医学基础与应用国家重点实验开放课题(SMFA19K06)
出版年
2024
国家科技期刊平台
NSTL
万方数据