摘要
目的:探讨白介素-1β(IL-1β)基因的单核苷酸多态性和单体型是否与原发性冻结肩的易感性相关，寻找原发性冻结肩的易感基因。方法:选取就诊于青岛市胶州中心医院的145名原发性冻结肩患者，排除继发性肩关节疼痛病人，冻结肩组患者男52例，女93例，年龄52～70岁；另收集了226例肩关节正常人作为对照组，男82例，女144例，年龄45～60岁。以聚合酶链反应和限制性片段长度多态性方法检测两组IL-1β基因的－511C/T(rs16944)、＋3954C/T(rs1143634)和－31C/T(rs1143627)位点的基因型，采用非条件logistic回归分析IL-1β不同位点基因型及三个位点基因形成的单体型与原发性冻结肩易感性的相关性。结果:经非条件logistic回归分析发现IL-1β rs1143634位点CT基因型在原发性冻结肩患者中比例明显增加，与对照组差异有统计学意义(P＝0.036)；杂合子IL-1β rs1143634CT基因型的患者发生PFS的风险是CC基因型的1.88倍[比值比(OR)＝1.88，95%置信区间( CI)(1.02，3.46)，P＝0.036]，同时IL-1βrs1143634位点的C基因是保护基因，在IL-1βrs1143634位点具备C基因发生原发性冻结肩的风险降低[OR＝0.542，95%CI(0.300，0.981)，P＝0.047]。IL-1βrs1143627位点CT基因型发生原发性冻结肩的风险是TT型的1.80倍，具有统计学意义[OR＝1.80，95%CI(1.09，2.99)，P＝0.019]。单体型分析发现：单体型TTT发生原发性冻结肩风险是CCT型的7.33倍[OR＝7.33，95%CI(1.66，32.28)，P＝0.02]。结论:IL-1β基因的rs1143634、rs1143627位点基因多态性与的原发性冻结肩的易感性相关。
Abstract
Objective:To investigate whether the single nucleotide polymorphism and haplotype of IL-1β gene are related to the susceptibility of primary frozen shoulder(PFS), and to search for the susceptibility gene of primary frozen shoulder.Methods:A total of 145 patients with PFS were selected from Jiaozhou Central Hospital of Qingdao, and secondary shoulder joint pain patients were excluded. PFS group included 52 males and 93 females, aged from 52 to 70 years. In addition, 226 normal shoulder joint volunteers were collected as the control group, including 82 males and 144 females, aged from 45 to 60 years. The genotypes of -511C/T (rs16944), + 3954C/T (rs1143634) and -31C/T (rs1143627) of IL-1β gene in two groups were detected by polymerase chain reaction and restriction fragment length polymorphism. The relationship between the haplotype of IL-β at different sites and the haplotype formed at three sites and the susceptibility to frozen shoulder was compared and analyzed by unconditional logistic regression.Results:Unconditioned logistic regression analysis showed that the proportion of rs1143634 CT genotype in the primary frozen shoulder was significantly increased, and the difference was statistically significant compared with the control group (P=0.036); patients with heterozygous rs1143634CT genotype had a risk of developing PFS 1.88 times greater than those with IL-1βrs1143634CC genotype [odds ratio(OR)=1.88, 95% confidence interval (CI )(1.02, 3.46), P=0.036]. At the same time, the C gene at rs1143634 was a protective gene, and the risk of primary frozen shoulder with C gene at rs1143634 was reduced[OR=0.542, 95%CI(0.300, 0.981), P=0.047]. CT genotype at rs1143627 had risk of primary frozen shoulder 1.80 times higher than TT genotype, which was statistically significant[OR=1.80, 95%CI (1.09, 2.99), P=0.019]. Haplotype analysis showed that the risk of primary frozen shoulder in haplotype TTT was 7.33 times than that of CCT type [OR=7.33, 95%CI(1.66, 32.28), P=0.02].Conclusion:rs1143634 and rs1143627 site polymorphisms of IL-1β gene are associated with susceptibility to primary frozen shoulder.
基金项目
青岛市医药卫生科研指导项目(2021-WJZD124)
万方数据