Objective To investigate the safety and effectiveness of the laparoscopic hepatectomy guided by three-dimensional visualization technology for treating hepatolithiasis in military flying personnel and to discuss the aeromedical assessment.Methods The diagnosis and treatment of a military flying personnel with hepatolithiasis admitted to the Air Force Medical Center in December of 2021 and the conclusions of aeromedical assessment were analyzed.The literature was reviewed in combination with the progress of new technologies.Results This case,a 40-year-old male helicopter aerial machinist,had been found gallbladder stones for 12 years,hepatolithiasis for 8 years,and intermittent right upper abdominal pain for more than 3 months.Ultrasound and CT examination showed multiple gallbladder stones,intrahepatic bile duct stones in the right posterior lobe of the liver with bile duct dilatation.Under the guidance of three-dimensional visualization technology,laparoscopic liver segment 6 precise resection,cholecystectomy,common bile duct exploration and stone removal,and T-tube drainage were performed.The patient was well recovered and waivered to flight after 6 months of the operation.As of the submission date,he has safely flown for 50 h.Conclusions Laparoscopic anatomical hepatectomy guided by three-dimensional visualization technology in the military flying personnel with hepatolithiasis shows good safety and effect,and those who recover well after surgery can be waivered to flight.