Objective To preliminarily evaluate the myocardium of pilots by noninvasive and radiation free cardiac MRI mapping technology,and to explore its application value in evaluating the myocardium of pilots.Methods Case-control study was adopted in this study.Pilots and normal personnel who had cardiac MRI in Air Force Medical Center from October of 2020 to July of 2022 were selected,and they were divided into 20-40 years group and>40 years group.The differences of myocardial longitudinal relaxation time T1,transverse relaxation time T2 and T2*values between pilots and normal personnel at different ages were analyzed and compared.Pilots were divided into groups according to flying hours and aircraft types,and the differences of myocardial T1,T2 and T2* values among pilots in different flying hours and aircraft types were analyzed and compared.Results The average myocardial T1 value of pilots[(1 011.18±24.70)ms]was lower than that of normal personnel[(1 025.07±25.74)ms],and the difference was significant(t=2.10,P=0.040).The myocardial T1 value of pilots>40 years old[(1 006.04±29.15)ms]was lower than that of normal personnel[(1 029.68±20.24)ms],and the difference was significant(t=2.30,P=0.032).The myocardial T1 value of fighter pilots[(999.04±17.39)ms]was lower than that of the bomber,transporter,helicopter pilots[(1 023.33±25.53)ms],and the difference was significant(t= 2.73,P=0.012).There was no significant difference in myocardial T1,T2 and T2* values between pilots and nornal personnel aged 20-40 years old;there was no significant difference in T2 and T2*values between pilots and normal personnel in the age group of>40 years old(all P>0.05).There was also no significant difference in myocardial T1,T2 and T2* values among the pilots in different flying hours(all P>0.05).Conclusions The level of cardiomyocyte hypertrophy or myocardial fibrosis in fighter pilots might be lower than that in other aircraft types pilots.Cardiac MRI mapping has potential value in evaluating the myocardium of pilots.