Diagnostic Value of ELISA and Chemiluminescence Detection of Hepatitis B Virus(HBV)
Objective To investigate the diagnostic value of chemiluminescence and enzyme-linked immunosorbent assay(ELISA)in hepatitis B virus.Methods Blood samples of 300 cases of viral hepatitis B and 300 cases of non-viral hepatitis B were collected in our hospital from June 2020 to April 2023.Hepatitis B virus(HBV)was detected by ELISA and chemiluminescence method in both groups.The results of the two groups were compared.Results The correlation coefficient R2=0.998 of the standard curve of hepatitis B virus(HBV)by chemiluminescence method,the average coefficient of intra-batch variation 2.98%,the coefficient of inter-batch variation 4.20%,The Log concentration of hepatitis B virus(HBV)detected by chemiluminescence method was(1.924±0.57)ng/mL in the case group and(0.108±0.03)ng/mL in the control group,with statistical difference.AUC of ROC curve in case group is 0.923.When the critical value is 11.86 ng/mL,the sensitivity and specificity were 95.28%and 92.30%,both higher than ELISA detection.The positive rate of chemical radiation group was also significantly higher than that of ELISA group.Conclusion Chemiluminescence is beneficial to the early diagnosis of viral hepatitis B,and has higher sensitivity and specificity than ELISA.
Hepatitis B virus(HBV)ELISA detectionChemiluminescence method
万方数据
维普
