Application Analysis of Parallel Blood Screening with Nucleic Acid Testing and Enzyme Immunoassay in Blood Stations
Objective To investigate effect of parallel blood screening in blood stations with nucleic acid testing and enzyme immunoassay.Methods The paper chose 15427 samples of voluntary blood donors in blood station for study,with parallel screening blood operations for each sample,and analyzed test results with detection techniques of nucleic acid amplification technology and enzyme-linked immunosorbent assay.Results ELISA showed that 15329 cases were HBsAg(hepatitis B surface antigen),anti HCV(hepatitis C virus antibody),anti HIV-1/2(AIDS virus type 1/AIDS virus type 2)non reactive and only one reagent reactive.Nucleic acid amplification technology found 98 out of 15329 samples were positive,with detection rate of 0.64%.After enzyme-linked immunosorbent assay(ELISA)testing,screening work was carried out for HB-sAg,anti HBs,anti HBe,HBeAg,anti HBc,etc.52 positive samples were detected,with confirmed positive rate of 53.06%.Quantitative testing of 98 positive samples with nucleic acid amplification technology showed,43 cases were HBV DNA positive,with positivity rate of 82.69%(43/52).However,no HIV RNA positive samples or HCV RNA positive samples were found.Conclusion Nucleic acid amplification technology and enzyme-linked immunosorbent assay can achieve complementary effect for parallel blood screening in blood station,can detect virus positive samples timely,improve quality of blood screening,reduce risk of viral disease transfusion transmission,and ensure blood safety.
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