Screening and Identification Research of NSCLC Plasma Biomarkers Based on UHPLC-MS Technology
Objective To study screening and identification of NSCLC plasma biomarkers with UHPLC-MS technology.Methods The paper chose 60 NSCLC patients met clinical diagnostic criteria for non-small cell lung cancer,and divided them into two groups randomly.Differential diagnosis group with non-small cell lung cancer patients was treated with targeted therapy(TACE),and normal control group with NSCLC patients with no targeted therapy.Results Among 60 NSCLC patients,552 biomarkers were detected with LC-MS/MS technology.Among the 17 biomarkers,8 appeared in known EGFR mutation patients,7 appeared in ALK fusion patients,and 6 appeared in HER2 mutation patients.Moreover,NSCLC patients had abundant non coding RNA(miRNA)in plasma,including 58 miRNAs and 23 mRNA.Conclusion The study analyzes plasma of NSCLC patients with LC-MS/MS technology,which can obtain biological markers in plasma samples quickly and efficiently.The biomarkers can serve as potential biological markers for diagnosis of NSCLC and can be used to predict tumor progression and poor prognosis.