Objective To explore value of passive agglutination and chemiluminescence methods in diagnose of mycoplasma pneumoniae(MP)infection of children.Methods The paper chose 53 children with respiratory tract infections in our hospital from April 2021 to December 2021 as research subjects,extracted 3ml of fasting venous blood,carried on detection with passive agglutination method and chemiluminescence method.Detection rate and diagnostic effect of dynamic agglutination method and chemiluminescence method for mycoplasma pneumoniae infection of children was analyzed with gold standard of immunofluorescence method.Results 43 cases of MP infection were detected among 53 children with respiratory tract infections,with detection rate of 43/53(81.13％),10 cases of non MP infection were detected,with detection rate of 10/53(18.87％).Chemiluminescence method showed good consistency with pathogen determination criteria(Kappa=0.870),while passive agglutination method showed acceptable consistency with pathogen determination criteria(Kappa=-0.537).Specificity(40.00％),sensitivity(69.77％),positive predictive value(83.33％),negative predictive value(23.53％)and diagnostic accuracy(64.15％)of chemiluminescence method for pneumonia MP infection was higher than passive agglutination method(P＜0.05).Conclusion Both passive agglutination and chemiluminescence methods can detect mycoplasma pneumoniae infection of children,and chemiluminescence detection can improve early detection and diagnosis rate of children with MP infection.
关键词
被动凝集法/化学发光法/儿童肺炎/支原体感染
Key words
Passive agglutination method/Chemiluminescence method/Pneumonia of children/Mycoplasma infection
维普
万方数据