Value Analysis of Enzyme-Linked Immunosorbent Assay and RT-PCR In Detecting Hepatitis B Virus of Blood Samples
Objective To explore the value of enzyme-linked immunosorbent assay (ELISA) and RT-PCR in detecting hepatitis B virus of blood samples. Methods The paper chose 78 patients with suspected hepatitis B in our hospital from January 2022 to October 2023 as research subjects,and detected Hepatitis B virus (HBV) serum markers and HBV-DNA contents with enzyme linked immunosorbent assay (ELISA) and real-time fluorescent quantitative PCR (RT-PCR). Those with simple ELISA test was included in the group A,and those with simple RT-PCR test in group B. With gold standard of final clinical diagnosis result,test results were analyzed,diagnostic efficacy was compared between two groups. Results In group A,the positive rate of HbeAg was 39.74％(31/78),that of HBsAg was 58.97％(46/78),that of HbcAb was 51.28％(40/78),that of HbeAb was 56.41％(44/78),that of HbsAb was 61.54％(48/78),that of HBsAb was 39.74％(31/78),that of HBsAg,HBeAg and HBcAb was 39.74％(31/78),that of HBsAg,HBeAb and HBcAb was 51.28％(40/78). In group B,the positivity rate of the HBV-DNA was 80.77％ (63/78). The diagnostic sensitivity,accuracy,and negative predictive value of group A for HBV were lower than group B (P<0.05),but the specificity and positive predictive value between two groups was comparable (P>0.05). Conclusion The RT-PCR method has higher value,sensitivity,and accuracy in detecting HBV in blood samples,which has higher overall diagnostic effect.
Blood samplesHepatitis B virusEnzyme linked immunosorbent assayReal time fluorescence quantitative PCRDiagnostic effect
万方数据
维普
