Correlation between obesity and difference of mRNA and protein expression of key factors of tumor necrosis factor-like weak inducer of apoptosis/fibroblast growth factor-induced early reactive protein 14 and downstream nuclear factor-kappa B pathway in the
Objective To study the correlation between central obesity and the difference of mRNA and protein expression of the tumor necrosis factor-like weak inducer of apoptosis(TWEAK)/fibroblast growth factor-induced early reactive protein 14(Fn14)and downstream nuclear factor-kappa B(NF-κB)pathway in peripheral blood mononuclear cells(PBMC)in the elderly.Methods From September 2017 to May 2018,80 subjects were randomly selected from the database of cross-sectional epidemiological survey of permanent residents in agricultural and pastoral areas of Xinjiang.According to whether they had central obesity or not,they were divided into the control group(n=40)and the central obesity group(n=40).PBMC were extracted from fasting peripheral blood,and the mRNA and protein expressions of TWEAK,Fn14,inhibitor of kappa B kinase-α(IKKα),inhibitor of kappa B kinase-β(IKKβ)and NF-κBp65 were detected by quantitative real-time polymerase chain reaction and Western-blotting.SPSS 26.0 was used for statistical analysis.Data comparison between two groups was performed using t-test or χ2 test,depending on data type.Correlation was analyzed using bivariate Pearson correlation analysis method.Results The mRNA expression of Fn14,IKK α,IKK β,TWEAK and NF-κBp65 in PBMC in the central obesity group was higher than that in the control group,but the difference was not statistically significant(P>0.05).The protein expression levels of Fn14,IKK α and IKK β in the central obesity group were higher than those in the control group(P<0.05),but there was no significant difference in TWEAK and NF-κBp65 protein expression between the two groups(P>0.05).Bivariate Pearson correlation analysis showed that mRNA expression of the above factors was positively correlated with TWEAK and Fn14(r = 0.472;P<0.01),Fn14 was positively correlated with IKK α and IKK β(r=0.262,0.275;P<0.05),and that IKK α and IKK β were positively correlated with NF-κBp65(r=0.747,0.692;P<0.01).Conclusion Central obesity in the elderly in the agricultural and pastoral areas of Xinjiang is related to the high expression of Fn14,IKK α,IKK β and mRNA in peripheral blood PBMC.TWEAK/Fn14 may be involved in the occurrence and development of human obesity by regulating IKK to activate NF-κB inflammatory pathway.
万方数据
维普
