Objective To investigate the distribution and the change of nicotine in blood and oral hard tissues of rats after passive smoking and abstaining from passive smoking,and indirectly confirm the harm of nicotine deposition in oral tissue through the effects of nicotine on the proliferation and osteogenic ability of PDLSCs in vitro.Methods The distribution of nicotine in blood,teeth and alveolar bone of rats was determined by liquid chromatography-tandem mass spectrometry(LC-MS).Different concentration of nicotine was used to stimulate PDLSCs,CCK-8,alizarin red staining and Western Blotting was used to detect the proliferation and osteogenic ability of PDLSCs respectively.Results During 3 months of passive smoking,nicotine concentrations in blood,alveolar bone,teeth peaked in February,January and gradually decreased,and nicotine levels were maintained after withdrawal from passive smoking.After in vitro nicotine stimulation,the proliferation of PDLSCs was significantly inhibited.The mineralized nodules were significantly reduced after osteogenic induction.WB results showed that the expression of proteins such as ALP,Runx2,OPG were all downregulated.Conclusion The distribution of nicotine in the blood,teeth and alveolar bone of passive smoking rats has a time correlation.After giving up passive smoking,nicotine may be accumulated in the body for a long time,causing adverse effects on the proliferation and osteogenic differentiation of PDLSCs.
关键词
被动吸烟/尼古丁/口腔硬组织/牙周病
Key words
Passive smoking/Nicotine/Oral hard tissue/Periodontal disease
维普
万方数据