Objective The pathological manifestations and structural changes of temporomandibular joint osteoarthritis in rats stimulated by monosodiumiodoacetate(MIA)were studied in order to find the appropriate concentration and time of action for temporomandibular joint osteoarthritis in rats.Methods A total of 24 6-week-old male Sprague-Dawley rats were randomly divided into 4 groups.Group 1(blank group)was injected with 0.9％normal saline.The other three groups were injected with different concentrations of MIA(0.5mg,1.0mg,and 2.0mg)into the TMJ,and the injection volume of each joint was 50pL.After 4 weeks of observation,all rats were sacrificed and the TMJ was analyzed.Hematoxylin eosin(HE)staining,Safranin O-Fast Green staining,TRAP staining and CD31 immunofluorescence staining were used to evaluate the pathological changes of temporomandibular joint tissues by modified Mankin's score.Results The results of HE and Safranin O-Fast Green staining showed that the condylar cartilage thickness,the number of chondrocyte layers were reduced,the arrangement was disordered,and the bone marrow cavity was enlarged in the 2.0 mg/50 uL group after injection of MIA1.0 mg/50 uL.No significant changes were observed in 0.5mg/50 uL,CD31 immunofluorescence staining showed that 1.0 mg/50 uL,2.0 mg/50 uL group showed cartilage and subchondral bone junction neovasculature,TRAP staining showed no activation of osteoclasts.MicroCT condylar scan showed the destruction of condylar cortex and cancellous bone only in the 2.0mg/50 uL group.Micro CT condylar scan showed the destruction of condylar cortex and cancellous bone only in the 2.0mg/50 uL group.Conclusion 1.0 mg/50 uL is the minimum effective dose of MIA to induce pathological changes in typical OA-like lesions of TMJ in rats,which can be used as an animal model for the preventive treatment of early organic lesions of TMJ.2.0mg/50uL or higher dose may induce typical OA-like changes in imaging and can be used for long-term observation as well as animal models of OA treatment.
维普
万方数据