Oct4基因在前列腺癌中的表达及意义
Expression and its clinical significance of Oct4 gene in prostate cancer
江明友 1万明发 1冯亮 1梅金红 1洪钟亮1
作者信息
- 1. 330006 浙江省桐庐县人民医院泌尿外科
- 折叠
摘要
目的 探讨Oct4在前列腺癌中的表达及临床意义. 方法 反转录-聚合酶链反应(RT-PCR)半定量分析2例正常前列腺和8例前列腺癌组织标本中Oct4 mRNA表达,SP免疫组织化学法检测10例正常前列腺、10例前列腺增生(BPH)和45例前列腺癌组织标本中Oct4蛋白表达水平. 结果 8例前列腺癌组织中Oct4 mRNA表达相对值分别为0.91、0.76、0.66、0.63、0.55、0.50、0.48、0.42,2例正常前列腺中相对值分别为0.正常前列腺、BPH和前列腺癌组织中Oct4蛋白阳性表达率分别为0.0％、0.0％和91.1％,前列腺癌组织中Oct4蛋白表达水平高于正常前列腺组织和BPH(P＜0.05).Oct4蛋白表达水平随前列腺癌临床分期、病理分级增高而增强.在有和没有淋巴结转移前列腺癌组织中Oct4强阳性表达率分别为72.7％、30.0％(P＜0.05). 结论 Oct4在前列腺癌组织中表达与分化程度、侵袭性关系密切,提示Oct与前列腺癌恶性程度有关,可能是评估前列腺癌预后的重要生物指标之一.
Abstract
Objective To study the expression and its clinical significance of Oct4 in prostate cancer.Methods The Oct4 mRNA expression levels in 2 normal prostate (NP) samples and 8 prostate cancer (Pca) samples were determined by reverse transcription polymerase chain reaction (RT-PCR) analysis.The Oct4 protein expression levels in 10 NP samples,10 benign prostate hyperplasia (BPH) samples and 45 Pca samples were detected by immunohisochemical analysis.Results The relative expression levels of Oct4 mRNA were 0.91,0.76,0.66,0.63,0.55,0.50,0.48,0.42 in 8 Pca samples and 0 in 2 NP samples,respectively.The positive rates of Oct4 protein expression was higher in Pca tissues than in NP and BPH tissues (91.1％ vs.0.0％,91.1％ vs.0.0％,both P＜0.05).The level of Oct4 protein was increased with higher hostologic grade and clinical stage of prostate cancer.The strong positive rates of Oct4 expression were 72.7％ and 30.0％ in Pca tissues with and without lymph node metastasis respectively,and there was a significant difference in the strong positive rate between them (P＜0.05).Conclusions The Oct4 expression is closely related to the differentiation and invasion of tumor.Oct4 might be an important index for the prognosis of prostate cancer.
关键词
前列腺肿瘤/肿瘤标记,生物学Key words
Prostatic neoplasms/Tumor markers,biological引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据