Expression and clinical value of miR-124 and miR-1976 in serum of patients with Parkinson's disease
Objective To investigate the expression and clinical significance of microRNA-124(miR-124)and microRNA-1976(miR-1976)in the serum of patients with Parkinson's disease(PD).Methods A total of 58 patients with PD were selected from September 2020 to June 2022 and categorized as the PD group.The Unified Parkinson's Disease Rating Scale(UPDRS)score was used to divide the PD patients into two groups:those with a UPDRS score≤60(25 patients)and those with a UPDRS score＞60(33 patients).The Hoehn-Yahr grading scale was used to grade the PD patients.Additionally,30 healthy individuals who had undergone a physical examination during the same period were selected as the control group.After collecting the subjects'serum,we performed real-time fluorescent quantitative PCR(qRT-PCR)to detect the expressions of miR-124 and miR-1976 in the serum.Logistic regression analysis was employed to analyze the influencing factors,and the diagnostic significance of serum miR-124 and miR-1976 in PD patients was evaluated using the receiver operating characteristic(ROC)curve.To predict the target genes of miR-1976,we utilized several software including TargetScan and Mirtarbase.Results Compared to the control group,the PD group showed a significant down-regulation of serum miR-124 expression[(1.49±0.36)vs.(1.02± 0.32)](t=8.85,P＜0.001),while miR-1976 expression was sharply up-regulated[(0.98±0.30)vs.(1.33±0.37)](t=6.92,P＜0.001).The low expression of serum miR-124 and the overexpression of miR-1976 were identified as independent risk factors for PD(OR＞1,P＜0.05).The Hoehn-Yahr rating of PD patients with a UPDRS score above 60 was higher than that of patients with a UPDRS score below 60[(3.42±0.73)vs.(2.16±0.42)](t=3.05,P＜0.05).However,there was no significant difference in serum miR-124 and miR-1976 expression between groups with different UPDRS scores[miR-124:(1.09±0.26)vs.(0.98±0.38)(t=0.89,P＞0.05);miR-1976:(1.42± 0.43)vs.(1.23±0.68)(t=0.62,P＞0.05)].The ROC analysis results demonstrated that miR-124 and miR-1976 had area under the curve(AUC)values of 0.832 and 0.797,respectively,in diagnosing PD.The corresponding cutoff values were 1.205 and 1.196,respectively.The sensitivity for miR-124 was 74.1％,while for miR-1976 it was 51.8％.The specificity for miR-124 was 77.8％,and for miR-1976 it was 90.1％.When both miR-124 and miR-1976 were combined in the diagnosis of PD,the AUC was 0.912,with a sensitivity of 76.4％and a specificity of 93.2％.Furthermore,it was found that miR-1976 targeted the PINK1 gene,suggesting its potential as a target gene in PD.Conclusions The expression of miR-124 was found to be decreased in PD patients,while the expression of miR-1976 was increased.Both miR-124 and miR-1976 showed some reference value in PD diagnosis,and their combined diagnostic value was higher.This suggests that further study on their significance is warranted.However,it should be noted that the expressions of miR-124 and miR-1976 were not found to be correlated with the UPDRS score of PD patients.
Parkinson's diseaseMicroRNA-124MicroRNA-1976UPDRS scoreClinical diagnosis value
NETL
NSTL
万方数据
维普
