The effect of levocarnitine on fibrotic proliferation,apoptosis and migration of myocardial cells
Objective To investigate the mechanisms underlying the effect of levocarnitine on myocardial cell fibrosis,proliferation,apoptosis and migration.Methods Between June and December 2022,an overexpression vector for tissue inhibitor-1 of metalloproteinase(TIMP-1)and siRNA TIMP-1 were used to transfect rat H9c2 cardiomyocytes(from the cell bank of the Chinese Academy of Sciences),and transfection efficiency was measured using fluorescence reverse transcription quantitative PCR(RT-qPCR).After treating H9c2 cells with angiotensin Ⅱ(AngⅡ),the expression of the MMP3 and TIMP-1 genes in the cells was detected by RT-qPCR.A CCK8 kit was used to assess the effect of levocarnitine intervention on the proliferation of myofibroblasts after overexpression or knockdown of TIMP-1.The effects of levocarnitine on apoptosis and migration of myofibroblasts were detected by flow cytometry and Transwell assays.Results The RT-qPCR results showed that the expression level of the MMP3 gene(1.38±0.05)in cardiomyocytes treated with AngⅡ for 24 hours exhibited an upward trend(P＜0.01),while the expression level of the TIMP-1 gene(0.71±0.03)showed a downward trend(P＜0.01).In addition,H9c2 cells with TIMP-1 overexpression(905.98±24.17)and knockdown(0.18±0.01)％,respectively,were successfully constructed.Based on CCK-8 detection results,knockdown of TIMP-1(86.56±7.98)％was able to promote the proliferation of H9c2 cells induced by levocarnitine(P＜0.01).Apoptosis experiments showed that inhibition of TIMP-1 expression(23.22±2.69)significantly reduced the apoptosis level of H9c2 cells induced by levocarnitine(P＜0.01).Migration experiments showed that inhibition of TIMP-1 expression(217.67±23.44)significantly promoted the migration ability of H9c2 cells induced by levocarnitine(P＜0.01).Conclusions After intervention to reduce TIMP-1 expression,levocarnitine can promote proliferation,inhibit apoptosis and promote migration of myofibroblasts and may therefore ameliorate myocardial fibrosis.
万方数据
维普
