Objective To observe the protective effect of Panax notoginseng total saponins(PNS)on oxidative stress damage induced by hydrogen peroxide(H2O2)in human renal tubular epithelial cells(HK-2)and its mechanism.Methods HK-2 was cultured and divided into experimental groups:Normal group(without any intervention factors),H2O2 group(400 μmol/L H2O2was added into the cell culture system for 6 h)and PNS group(which was divided into three groups:PNS 25 μg/ml,50 μg/ml and 100 μg/ml were added into the cell culture system for 4 h,and then 400 μmol/l H2O2 was added into the cell culture system for 6 h),and the apoptosis of HK-2 induced by H2O2 was observed.Results Compared with the normal group,the cell survival rate of H2O2 group decreased to(45.67±2.52)％and the cell apoptosis rate increased to(19.23±1.63)％(all P＜0.01),while the cell survival rate of PNS group was(55.12±2.33)％,(65.37±4.72)％and(83.46±1.52)％,and the apoptosis rate was reduced to(15.53±0.70)％,(12.53±1.30)％and(7.17±0.35)％,respectively.The levels of reactive oxygen species(ROS)in HK-2 cells in 50 μg/ml and 100 μg/ml PNS groups were(845.7±17.79)and(353.3±26.1),respectively.With the increase of PNS dose,super oxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities increased and malonic dialdehyde(MD A)production decreased in the 3 PNS groups,compared with the H2 O2 group,the differences were statistically significant(all P＜0.01).Conclusions PNS can protect HK-2 cells against oxidative damage by enhancing the activity of intracellular antioxidant enzymes,the effect is enhanced with the increase of the dose.
维普
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