Panax notoginseng saponins alleviated oxidative stress damage of renal tubular epithelial cells induced by hydrogen peroxide
Objective To observe the protective effect of Panax notoginseng total saponins(PNS)on oxidative stress damage induced by hydrogen peroxide(H2O2)in human renal tubular epithelial cells(HK-2)and its mechanism.Methods HK-2 was cultured and divided into experimental groups:Normal group(without any intervention factors),H2O2 group(400 μmol/L H2O2was added into the cell culture system for 6 h)and PNS group(which was divided into three groups:PNS 25 μg/ml,50 μg/ml and 100 μg/ml were added into the cell culture system for 4 h,and then 400 μmol/l H2O2 was added into the cell culture system for 6 h),and the apoptosis of HK-2 induced by H2O2 was observed.Results Compared with the normal group,the cell survival rate of H2O2 group decreased to(45.67±2.52)％and the cell apoptosis rate increased to(19.23±1.63)％(all P＜0.01),while the cell survival rate of PNS group was(55.12±2.33)％,(65.37±4.72)％and(83.46±1.52)％,and the apoptosis rate was reduced to(15.53±0.70)％,(12.53±1.30)％and(7.17±0.35)％,respectively.The levels of reactive oxygen species(ROS)in HK-2 cells in 50 μg/ml and 100 μg/ml PNS groups were(845.7±17.79)and(353.3±26.1),respectively.With the increase of PNS dose,super oxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities increased and malonic dialdehyde(MD A)production decreased in the 3 PNS groups,compared with the H2 O2 group,the differences were statistically significant(all P＜0.01).Conclusions PNS can protect HK-2 cells against oxidative damage by enhancing the activity of intracellular antioxidant enzymes,the effect is enhanced with the increase of the dose.
万方数据
维普
