首页|miR-182-5p调控初级纤毛的缺失增加甲状腺乳头状癌细胞系TPC-1的迁移

miR-182-5p调控初级纤毛的缺失增加甲状腺乳头状癌细胞系TPC-1的迁移

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目的 探讨miR-182-5p调控初级纤毛的缺失对甲状腺乳头状癌(PTC)细胞系TPC-1迁移的影响。 方法 收集10例兰州大学第一医院PTC组织和癌旁组织,qPCR检测癌旁组织、PTC组织和TPC-1细胞中miR-182-5p的表达量,免疫荧光检测初级纤毛的形成;过表达miR-182-5p,qPCR和Western blot检测TPC-1细胞中PI3K的表达量,Transwell实验检测TPC-1细胞迁移数量,免疫荧光检测初级纤毛的形成;siRNA-IFT88干扰TPC-1细胞,Transwell实验检测TPC-1细胞迁移数量,免疫荧光检测初级纤毛的形成,吉姆萨染色检测形态学变化,Western blot检测E-cadherin和N-cadherin的蛋白表达量。 结果 与癌旁组织相比,PTC组织中初级纤形成受损;miR-182-5p表达量显著上调。与Nthy-ori3-1相比,TPC-1细胞中初级纤毛的频率减少;miR-182-5p表达量显著上调(P=0.001);miR-182-5p mimic治疗后的TPC-1细胞中PI3K的表达量降低,迁移数量增加(P=0.001),初级纤毛频率降低为27%(P=0.002);siRNA-IFT88治疗后的TPC-1细胞表面初级纤毛变短变细,频率减少(P=0.001),迁移数量增加(P<0.001)。 结论 miR-182-5p通过PI3K通路调控初级纤毛的缺失有助于TPC-1细胞的迁移,初级纤毛的丢失对PTC预后具有不利的影响。 Objective To investigate the role of miR-182-5p regulated primary cilia loss on migration of TPC-1 in papillary thyroid cancers(PTC). Methods Ten cases of PTC and adjacent tissues were collected from the First Hospital of Lanzhou University, The expression of miR-182-5p in PTC tissue and TPC-1 cells was detected by qPCR, and the frequency of primary cilia was detected by immunofluorescence Overexpressing miR-182-5p, the migrated number of TPC-1 was detected by Transwell assay Interfering TPC-1 with siRNA-IFT88, the migrated number of TPC-1 and the frequency of primary cilia were detected, respectively. Results Compared with control, the expression of miR-182-5p was significantly upregulated in PTC and TPC-1, the frequency of primary cilia in PTC and TPC-1 was downregulated. Overexpressing miR-182-5p increased the migrated number of TPC-1 cell and reduced the number of TPC-1 cell migration(27%, P=0.002) After siRNA-IFT88 treatment, primary cilia in TPC-1 became shorter and thinner, with a decrease in frequency(P=0.001), the migrated number of TPC-1 cell increased, and TPC-1 cell showed smaller nuclei and fewer microvilli. Conclusion The regulation of primary cilia loss by miR-182-5p through the PI3K pathway contributes to the migration of TPC-1 cells. The loss of primary cilia has an adverse impact on the prognosis of PTC.
Loss of primary cilia promotes migration of TPC-1 in papillary thyroid cancers
Objective To investigate the role of miR-182-5p regulated primary cilia loss on migration of TPC-1 in papillary thyroid cancers(PTC). Methods Ten cases of PTC and adjacent tissues were collected from the First Hospital of Lanzhou University, The expression of miR-182-5p in PTC tissue and TPC-1 cells was detected by qPCR, and the frequency of primary cilia was detected by immunofluorescence Overexpressing miR-182-5p, the migrated number of TPC-1 was detected by Transwell assay Interfering TPC-1 with siRNA-IFT88, the migrated number of TPC-1 and the frequency of primary cilia were detected, respectively. Results Compared with control, the expression of miR-182-5p was significantly upregulated in PTC and TPC-1, the frequency of primary cilia in PTC and TPC-1 was downregulated. Overexpressing miR-182-5p increased the migrated number of TPC-1 cell and reduced the number of TPC-1 cell migration(27%, P=0.002) After siRNA-IFT88 treatment, primary cilia in TPC-1 became shorter and thinner, with a decrease in frequency(P=0.001), the migrated number of TPC-1 cell increased, and TPC-1 cell showed smaller nuclei and fewer microvilli. Conclusion The regulation of primary cilia loss by miR-182-5p through the PI3K pathway contributes to the migration of TPC-1 cells. The loss of primary cilia has an adverse impact on the prognosis of PTC.

Papillary thyroid carcinomaMigrationPrimary ciliamiR-182-5pTPC-1

马承旭、马小妮、马丽华、傅松波、朱鋐达

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兰州大学第一临床医学院,兰州大学第一医院内分泌科,兰州 730000

甲状腺乳头状癌 迁移 初级纤毛 miR-182-5p TPC-1

甘肃省内分泌疾病临床医学研究中心

20JR10FA667

2024

中华内分泌代谢杂志
中华医学会

中华内分泌代谢杂志

CSTPCD北大核心
影响因子:1.747
ISSN:1000-6699
年,卷(期):2024.40(2)
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