目的 探索辅酶Q10(coenzyme Q10, CoQ10)对高脂膳食诱导的小鼠肥胖、脂质紊乱及胆汁酸代谢的影响。 方法 8周龄C57BL/6J小鼠根据体重按照随机区组法随机分为对照组(普通饲料)、高脂膳食组(45%高脂饲料)和CoQ10干预组(45%高脂饲料＋100 mg·kg－1·d－1 CoQ10)。收集各组小鼠体重、摄食量数据；试剂盒检测血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、丙氨酸转氨酶和天冬氨酸转氨酶水平；超高效液相色谱串联质谱(UPLC-MS/MS)检测小鼠血清、肝脏和结肠中17种胆汁酸的含量，Western印迹检测肝脏中胆固醇12α-羟化酶(cholesterol 12α-hydroxylase, CYP8B1)和氧固醇7α-羟化酶(oxysterol 7α-hydroxylase, CYP7B1)蛋白的表达。 结果 CoQ10显著降低了高脂膳食小鼠的体重并改善其脂质代谢紊乱。与对照组相比，高脂膳食组小鼠血清总胆汁酸水平降低(P<0.05)；与高脂膳食组相比，CoQ10干预升高了小鼠血清和结肠中的总胆汁酸水平(P＝0.021, P＝0.014)，并提高了小鼠肝脏、结肠和血清中脱氧胆酸和熊脱氧胆酸的水平(P<0.05)。CoQ10干预组小鼠结肠和血清脱氧胆酸水平均与小鼠体重呈负相关(P＝0.024, P＝0.019)，结肠脱氧胆酸和总胆固醇水平也呈负相关(P＝0.006)。CoQ10上调了高脂膳食小鼠肝脏中CYP8B1和CYP7B1蛋白的表达。 结论 CoQ10能够调节高脂膳食小鼠的胆汁酸代谢并减轻其肥胖及脂质代谢紊乱。 Objective To explore the effects of coenzyme Q10(CoQ10) on high-fat diet-induced obesity, lipid disorders, and bile acid metabolism in mice. Methods Eight-week-old C57BL/6J mice were randomly divided into control group(regular chow), high-fat diet group(45% high-fat chow), and CoQ10 intervention group(45% high-fat chow+ 100 mg·kg-1·d-1CoQ10) based on their body weights according to the randomized block design. The body weight and food intake of mice in each group were collected. The levels of serum total cholesterol, triglyceride, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, alanine aminotransferase, and aspartate aminotransferase were detected. The contents of 17 bile acids in serum, liver, and colon contents of mice were detected by ultra-performance liquid chromatography-tandem mass(UPLC-MS/MS). The protein expressions of cholesterol 12α-hydroxylase(CYP8B1) and oxysterol 7α-hydroxylase(CYP7B1) in liver were detected by Western blotting. Results CoQ10 significantly reduced body weight and ameliorated lipid metabolism disorders in mice fed a high-fat diet. Compared with the control group, serum total bile acid levels were reduced in the high-fat diet group(P<0.05) CoQ10 intervention elevated serum and colonic total bile acid levels(P=0.021, P=0.014) and increased liver, colon, and serum deoxycholic acid and ursodeoxycholic acid levels(P<0.05) in the mice compared with the high-fat diet group. Both colonic and serum deoxycholic acid levels in the CoQ10 intervention group were negatively correlated with body weights(P=0.024, P=0.019), and colonic deoxycholic acid and total cholesterol levels were also negatively correlated(P=0.006). CoQ10 increased the expression of CYP8B1 and CYP7B1 proteins in the liver of mice. Conclusion CoQ10 can modulate bile acid metabolism in high-fat diet-fed mice and alleviate their obesity and lipid metabolism disorders.
Effects of coenzyme Q10 on bile acid metabolism, obesity, and related lipid metabolism disorders in high-fat diet mice
Objective To explore the effects of coenzyme Q10(CoQ10) on high-fat diet-induced obesity, lipid disorders, and bile acid metabolism in mice. Methods Eight-week-old C57BL/6J mice were randomly divided into control group(regular chow), high-fat diet group(45% high-fat chow), and CoQ10 intervention group(45% high-fat chow+ 100 mg·kg-1·d-1CoQ10) based on their body weights according to the randomized block design. The body weight and food intake of mice in each group were collected. The levels of serum total cholesterol, triglyceride, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, alanine aminotransferase, and aspartate aminotransferase were detected. The contents of 17 bile acids in serum, liver, and colon contents of mice were detected by ultra-performance liquid chromatography-tandem mass(UPLC-MS/MS). The protein expressions of cholesterol 12α-hydroxylase(CYP8B1) and oxysterol 7α-hydroxylase(CYP7B1) in liver were detected by Western blotting. Results CoQ10 significantly reduced body weight and ameliorated lipid metabolism disorders in mice fed a high-fat diet. Compared with the control group, serum total bile acid levels were reduced in the high-fat diet group(P<0.05) CoQ10 intervention elevated serum and colonic total bile acid levels(P=0.021, P=0.014) and increased liver, colon, and serum deoxycholic acid and ursodeoxycholic acid levels(P<0.05) in the mice compared with the high-fat diet group. Both colonic and serum deoxycholic acid levels in the CoQ10 intervention group were negatively correlated with body weights(P=0.024, P=0.019), and colonic deoxycholic acid and total cholesterol levels were also negatively correlated(P=0.006). CoQ10 increased the expression of CYP8B1 and CYP7B1 proteins in the liver of mice. Conclusion CoQ10 can modulate bile acid metabolism in high-fat diet-fed mice and alleviate their obesity and lipid metabolism disorders.
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