白藜芦醇提高多囊卵巢综合征大鼠卵巢局部抗氧化水平的研究
Resveratrol enhances the local antioxidant ability of the ovary in rats with polycystic ovary syndrome
沈涛 1梁元姣 2招霞 2卢苏3
作者信息
- 1. 南京中医药大学第一临床医学院,江苏南京 210004;东南大学附属中大医院生殖医学科,江苏南京 210004
- 2. 东南大学附属中大医院生殖医学科,江苏南京 210004
- 3. 南京中医药大学第一临床医学院,江苏南京 210004
- 折叠
摘要
目的:探讨白藜芦醇(RSV)对多囊卵巢综合征(PCOS)大鼠卵巢形态、血浆抗苗勒管激素(AMH)、胰岛素样生长因子1(IGF-1)水平及氧化应激参数的影响.方法:采用注射脱氢表雄酮方法构建PCOS大鼠模型,将大鼠分为对照组(n=12)、模型组(n=12)、罗格列酮组(RSG组,n=11)和白藜芦醇组(RSV组,n=11)o RSG组给予3.0 mg/(kg·d)的RSG灌胃,RSV组给予10 mg/(kg·d)的RSV灌胃,模型组和对照组均给予等量生理盐水灌胃,连续用药4周.以光学显微镜观察卵巢组织学,以酶联免疫吸附法检测血浆AMH和IGF-1水平,以Ellman法检测卵巢组织谷胱甘肽过氧化物酶(GSH-Px)活性,以Sun法检测卵巢组织超氧化物歧化酶(SOD)活性,以AEBI法测定卵巢组织过氧化氢酶(CAT)活性.结果:给药4周后,模型组与对照组相比较,上述指标差异均有统计学意义(P＜0.05),RSG和RSV组与模型组相比较,上述指标差异均有统计学意义(P＜0.05).结论:RSV可提高PCOS大鼠卵巢局部抗氧化能力,降低IGF-1和AMH水平,改善PCOS大鼠卵巢组织形态,提示其治疗PCOS的潜在价值.
Abstract
Objective:To investigate the effects of resveratrol(RSV)on ovarian morphology,plasma anti-Müllerian hormone(AMH)and insulin-like growth factor 1 levels(IGF-1),and oxidative stress parameters in rats with polycystic ovary syndrome(PCOS).Methods:Forty-six rats were randomly divided into a normal control(n=12),a PCOS model control(n=12),a ros-iglitazone(RSG,n=11),and an RSV group(n=11).The PCOS model was established in the latter three groups by rejection of epidehydroandrosterone.The rats in the normal control and PCOS model control groups were treated by gavage of normal saline and those in the RSG and RSV groups by intragastric administration of RSG at 10 mg/(kg·d)and RSV at 3.0 mg/(kg·d),respective-ly.After 4 weeks of treatment,the ovarian histology was observed under the light microscope,the levels of plasma AMH and IGF-1 measured by ELISA,and the activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD)and catalase(CAT)in the ovarian tissue detected using the Ellman,Sun and AEBI methods,respectively.Results:After a4-week treatment,statistically sig-nificant differences were observed in the above indicators between the normal control and PCOS model control groups(P＜0.05).The rats treated with RSG and RSV also showed significant differences in these parameters from the model controls(P＜0.05).Conclu-sion:RSV can enhance the local antioxidant capacity of the ovary,reduce the levels of AMH and IGF-1,and improve the morphology of the ovarian tissue in rats with PCOS,indicating its potential value in the treatment of PCOS.
关键词
抗苗勒管激素/谷胱甘肽过氧化物酶/多囊卵巢综合征/白藜芦醇/超氧化物歧化酶Key words
anti-Müllerian hormone/glutathione peroxidase/polycystic ovary syndrome/resveratrol/superoxide dismutase引用本文复制引用
基金项目
东南大学附属中大医院人才引进基金(ZDYYZXKT2019001)
出版年
2024
NETL
NSTL
万方数据