首页|弱精子症患者精子lncRNA与mRNA基因表达特征分析

弱精子症患者精子lncRNA与mRNA基因表达特征分析

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目的:通过DNBSEQ平台测序技术构建文库,并以此来确定人类正常与弱精子症精子中lncRNA和mRNA的表达差异,并通过生物信息学方法分析其在弱精子症中的生物学意义.方法:将9份正常精液样本及9份弱精子症精液样本分组后,分离精子,提取总RNA,通过DNBSEQ测序平台检测得到RNA-seq在精子中的表达情况,通过GO富集分析和KEGG通路分析,进一步分析其相关功能情况.结果:使用DNBSEQ平台检测,每组平均产出10.64G数据,共检测到282 185个RNA,包含有107 009个lncRNA,其中15 157个lncRNA具有表达差异,2 190个lncRNA表达上调,12 967个表达下调;共检测到19 514个mRNA,其中13 736个mRNA具有表达差异,4 995个mRNA表达上调,8 741个mRNA表达下调.差异基因主要富集在精子细胞膜、离子通道的功能以及精子发育、受精相关的通路上.结论:通过对弱精子症与正常精子的测序分析,鉴别出差异表达的lncRNA和mRNA,可通过胞膜离子通道对精子功能进行调控,进而导致弱精子症的发生,可为弱精子症的进一步研究提供分子基础.
Gene expression characteristics of lncRNAs and mRNAs in the sperm of asthenospermia patients
Objective:To determine the differential expressions of long non-coding RNA(lncRNA)and messenger RNA(mRNA)in normal and asthenospermia(AS)men and analyze their biological significance in AS.Methods:We isolated and ex-tracted total RNAs from 9 normal and 9 AS sperm samples,determined the expressions of RNAs in the sperm using the DNBSEQ se-quencing platform,and analyzed their relevant functions by gene ontology enrichment(GO)and Kyoto encyclopedia of genes and ge-nomes pathway(KEGG)analyses.Results:An average of 10.64G data was generated per group,with 282 185 RNAs detected,in-cluding 107 009 lncRNAs.Among the total number of lncRNAs,15 157 were differentially expressed,2 190 upregulated and 12 967 downregulated;and among the 19 514 mRNAs,13 736 were differentially expressed,4 995 upregulated and 8 741 downregulated.Differentially expressed genes were enriched mainly in the sperm cell membrane and the pathways related to the ion channel functions,sperm development and fertilization.Conclusion:Differentially expressed lncRNAs and mRNAs can be identified by sequencing a-nalysis of AS and normal sperm.Regulation of sperm function through membrane ion channels may contribute to the development of AS,which provides a molecular basis for further research on AS.

long non-coding RNAmessenger RNAasthenozoospermiaRNA sequencingdifferential expression

石水波、罗龙华、刘莲、黄学明、熊素萍、宋丹丹、李冬水

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南昌大学第一附属医院男科,江西南昌 330006

长链非编码RNA 弱精子症 RNA测序 差异表达分析

江西省自然科学基金项目

20202BAB206014

2024

中华男科学杂志
南京军区南京总医院

中华男科学杂志

CSTPCD
影响因子:1.052
ISSN:1009-3591
年,卷(期):2024.30(9)
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