Impacts of varicocele on the structure and proteomics of rat testis tissue:An experimental study
Objective:To study the impacts of varicocele(VC)and varicocelectomy(VCT)on the proteomics of rat testis tis-sue,and to analyze the differential proteins and signaling pathways,and observe the microstructural changes of the testis tissue.Methods:We selected 60 male SD rats and divided them into a sham operation(SO),a VC model control,and a VCT group.We harvested the testis tissues from the rats at 4 weeks after modeling for determination of the differential protein expressions by mass spec-trometry,analysis of the changes in the protein signaling pathways by KEGG pathway repolarization,and observation of the microstruc-tural changes in the spermatogenic cells under the transmission electron microscope(TEM).Results:A total of 15 clinically signifi-cant proteins were effectively identified,among which RPS24,KIFAP3,HPX,RPL38,TOP2A,PRPF19,TRPM3,RPL32,CNBP and AHSG were upregulated,while RPS9,TKFC,SH3BGRL3,ACAA2 and FABP3 downregulated.The differential pathways found included the Type-Ⅰ 4-aminobutyrate degradation pathway,eIF2 signaling pathway,and Type-Ⅲ glutamate degradation pathway,which were all related to the pathogenesis of testicular growth arrest.Compared with the rats in the VCT group,those of the VC group rats showed ultrastructural changes in the testis tissue under the TEM,such as mitochondrial vacuolar degeneration,dense nucleoli,invagi-nation of cell nuclear membranes,and irregularity,which were detrimental to the survival of testicular cells.Conclusion:VCT af-fects the development and growth of the testis by altering the expressions of relevant proteins and influencing the changes of the gene pathways in the testicular cells,which may be one of the causes of VC inducing testis injury and testicular spermatogenic dysfunction.The changes in these molecular pathways can provide some theoretical evidence for an insight VC as well as potential therapeutic targets for its treatment.
varicocelehigh ligation of spermatic veintestis tissuedifferential proteinsrat
万方数据
维普
