This study carried out the research and application of blended teaching of Animal Pharmacology using Xueyin Online as a platform.Online courses are established in Xueyin online,and the course resources include 18 lecture videos,152 minutes of teaching videos,36 non-video resources,53 course materials,and so on.The blended teaching practice was carried out on 256 students from 7 classes,and the blended teaching mode was carried out in front of class virtual classroom,middle and off-line class,and after-class off-line class.The total number of participants was 601,the total number of participants in the questionnaire was 426,and the number of participants was 256.All 256 students passed the course examination and got credits,of which more than 90 points accounted for 5.47%,80-89 points accounted for 28.91%,70-79 points accounted for 46.09%,and 60-69 points accounted for 19.53%.