Rural tourism is becoming an important way to help the optimization and transformation of rural industry,solve the lack of motivation for rural development,and solve the employment of rural workers nearby.Local culture in ethnic areas is the key point and breakthrough for the high-quality development of rural tourism.On the basis of literature review,the paper designs the technical path of cultural gene clustering"decoding"in rural tourism destinations,and clusters the rural tourism projects in Huaihua City,Hunan Province layer by layer according to the technical path.It is found that five cultural genes are the cultural core of the development of rural tourism in this region,occupy the core position of rural tourism products,and become the core attraction of rural tourism.The future is also the cultural gene for the high-quality development of rural tourism in this region,which can promote the high-quality development of rural tourism in four ways.This study can point out a new direction and inject new power for the high-quality development of rural tourism,and provide reference and reference for other areas.