In view of the characteristics of plant tissue culture courses and the problems existing in the construction of teaching materials,this paper puts forward specific reform strategies for the construction of teaching materials for plant tissue culture based on the concept of OBE,and carries out practice and exploration.The teaching material of plant tissue culture is jointly developed by schools and enterprises and compiled based on the working process.Based on the talent training plan and post ability requirements of this major,it is proposed to update the relevant contents in time,so as to rebuild the curriculum system;to realize the innovation in textbook form through the combination of theoretical teaching materials and training manuals;and to organically integrate the situation of production,learning and research in our university,in order to realize the practicality and localization of teaching content.