Biochar is commonly used for soil amendment in citrus orchards across Guangxi,where acidic soils are prevalent and nitrogen transformation is characterized by its specificity.However,the impact of biochar application on the microbial communities involved in nitrogen fixation remains unclear.This study focused on the microbial communities of nitrogen-fixing bacteria in the acidic soils of navel orange orchards in Guangxi.Through high-throughput sequencing and bioinformatics analysis,it was found that the nitrogen-fixing microbial community in these soils encompasses 50 genera within 39 families,28 orders,15 classes,and 8 phyla.The relative abundance of Proteobacteria increased following biochar treatment,and the highest OTU numbers,Ace index,and Chao index were observed in the group with a 5%biochar input,although differences in diversity indices among groups were not significant.PcoA and NMDS analyses demonstrated that biochar application significantly altered the structure of the soil nitrogen-fixing bacterial community,with the degree of change being related to the amount of biochar added.Soil pH,NH4+-N content,and mineralization rate were identified as the main environmental factors influencing the structure of the nitrogen-fixing bacterial community in the acidic soils of navel orange orchards,with different populations of nitrogen-fixing bacteria responding differently to environmental factors.
万方数据
维普
