To establish a method for the determination of Zanthoxytoxin in Glehniae Radix. Ultra-performance liquid chromatog-raphy was used. The column was ACQUITY UPLC(R) HSS T31.8μm (2.1X100 mmX1.8μm),the mobile phase was 50％ methanol acetonitrile-0.1％ phosphoric acid solution,the flow rate was 0.3 mL/min,the column temperature was 30 ℃,the detection wave-length was 323 nm,and the injection volume was 1μL. Under the chromatographic conditions,there was a good linear relationship between the concentration of Zanthoxytoxin and peak area in the range of 2.005～80.217 μg/mL (R=0.9998). The method precision RSD was 0.23％,the repeatability RSD was 0.87％,the stability RSD was 0.91％,and the sample addition recovery was between 97.0％～105.5％(RSD=2.6％). The method is rapid,accurate and convenient,and thereby can be applied to determine the content of Zanthoxytoxin in Glehniae Radix.
ultra-performance liquid chromatographyGlehniae RadixZanthoxytoxincontent determinationcurrent research
万方数据
维普
