Effect of Kanglaite Injection associated with Cisplatin in lung adno carcinoma cells A549
AIM:To investigate the effect and mechanism of Kanglaite Injection associated with Cisplatin(CD-DP) in lung adno carcinoma cells A549. METHODS:The lung adno carcinoma cells A549 was treated by Kanglaite Injection associated with CDDP in different groups, then inhibition rates were measured by SRB assay in vitro. The expressions of MDR1/P-gp, Bcl-2, and Bax were determined by reverse transcription-polymerase chain reaction and Western blot in A549 cells. The effect of Kanglaite on human brain microvascular endothelial cells (HB-MEC) was also tested. RESULTS:The survival rate of A549 cells treated by Kanglaite was lower than that of the control group ( all P < 0.05 ) , and the survival rate decreased more when Kanglaite combined with CDDP;Kanglaite Injection had no obvious inhibition on HBMEC. The levels of MDRl/P-gp, Bcl-2 (mRNA and proteins)were lower in cells after being treated by Kanglaite( all P < 0. 05 ) , but expression of Bax was higher( P < 0. 05 );also Kanglaite had no obvious influence on these factors in HBMEC ( all P > 0.05 ). CONCLUSION:Kanglaite can inhibit the growth of A549 cells, and it also can reverse multidrug resistance of A549 by regulating the expression of multidrug resistance associated factors. Combination of Kanglaite and CDDP can increase chemosensitivity of A549 cells.
lung neoplasmsKanglaitechemotherapy, Cisplatin(CDDP)chemosensitivity test(SRB assay) in vitro
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