首页|HPLC-ELSD法测定血塞通注射液中三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rd

HPLC-ELSD法测定血塞通注射液中三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rd

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目的 采用HPLC-ELSD法同时测定血塞通注射液(三七)中三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rd.方法 采用Venusil XBP C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水梯度洗脱,体积流量1 mL/min,柱温30℃,雾化管温度36℃,漂移管温度70℃,载气压力25 psi(1 psi=6.895 kPa).结果 三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rd分别在2.78～27.8 μg/mL,9.24～92.4 μg/mL,0.384 ～ 3.84 μg,/mL,5.55～55.5 μg/mL,1.904～ 19.04 μg/mL呈良好线性关系；血塞通注射液中三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rd平均回收率(n=6)分别为97.60％、100.71％、98.14％、100.94％、99.69％.结论 该方法简便、准确、分离好,灵敏度高,重复性好,无干扰,可用于血塞通注射液的质量评价及三七制剂的质量控制.
Determination of notoginsenoside R1 and ginsenoside Rg1, Re, Rb1, Rd in Xuesaitong Injection by HPLC-ELSD
AIM To establish a method for simultaneously determining notoginsenoside R1 and ginsenoside Rg1, Re, Rb1, Rd in Xuesaitong Injection (Notoginseng Radix et Rhizoma) by HPLC-ELSD. METHODS A Venusil XBP C18 column (4. 6 mm ×250 mm, 5 μm) was used with the mobile phase of acetonitrile-water for notoginsenoside R1, ginsenoside Rg1, Re, Rb1, Rd. The flow rate was 1. 0 mL/min at 30℃. The temperature of driti tube was 70℃ and the nebulizer nitrogen flow was 25 psi (1 psi =6. 895 kPa). RESULTS The linearities of notoginsenoside R1, ginsenoside Rg1, Re, Rb1, Rd were in the ranges of 2. 78 -27. 8 μg/mL, 9. 24 -92. 4 μg/ mL, 0. 384 -3. 84 μg/mL, 5. 55 -55. 5 μg/mL and 1. 904 - 19. 04 μg/mL, respectively. The average recoveries were 97. 60% , 100.71% , 98. 14% , 100.94% and 99.69% , respectively. CONCLUSION The method is simple, reliable and precise, and could be used for quality control of the injection.

HPLC-ELSDXuesaitong Injectionnotoginsenoside R1ginsenoside Rg1ginsenoside Reginsenoside Rb1ginsenoside Rd

徐鹏、冯素香、赵迪、李娟、高聪聪、周悌强

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河南中医学院,河南郑州450008

HPLC-ELSD 血塞通注射液 三七皂苷R1 人参皂苷Rg1 人参皂苷Re 人参皂苷Rb1 人参皂苷Rd

国家自然科学基金重大项目

81130062

2013

10.3969/j.issn.1001-1528.2013.03.021

中成药
国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCDCSCD北大核心
影响因子：1.217
ISSN：1001-1528
年,卷(期)：2013.35(3)
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