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毛蒟挥发油测定及其贮存期考察

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  • 国家科技期刊平台
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  • NSTL
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  • 维普
目的 水蒸汽蒸馏法提取广西产毛蒟中挥发油,GC-MS法测定挥发油中β-石竹烯含有量,并对其最佳贮存期进行考察.方法 挥发油的分析色谱柱为HP-5MS (30 m×0.25 mm,0.25 μm);程序升温;载气为氦气;水杨酸甲酯为内标物.结果 挥发油得油率在0.23％～1.64％之间,贮存1年后其下降率为22.68％,1.5年后下降率为41.63％;β-石竹烯在0.033 9～0.542 4μg范围内与峰面积呈良好的线性关系(r=0.999 6),平均回收率为98.5％ (RSD=2.0％,n =6);β-石竹烯含有量在31.363 8 ～338.957 2 mg/mL之间,一年后其下降率为35.03％,一年半后下降率为44.53％.结论 建议毛蒟的贮存时间不超过1年.
Determination of volatile oil in Piper puberulum and its proper storage time
AIM To determine β-caryophyllene content in volatile oil isolated from Piper puberulum by GCMS choose its proper storage time.METHODS The analysis of volatile oil was performed on HP-5MS (30 m ×0.25 mm,0.25 μm) column with temperature-programming mode,carrier gas was He,and internal standard substance was methyl salicylate.RESULTS Volatile oil rate was between 0.23％ and 1.64％.A year later,its drop rate was 22.68％.After a year and a half,it was 41.63％.There was a good linear relationship for β-caryophyllene during 0.033 9-0.542 4 μg (r =0.999 6),the average recovery rate was 98.5％ (RSD =2.0％,n =6),the content of β-caryophyllene was 31.363 8-338.957 2 mg/mL.A year later,its drop rate was 35.03％.After a year and a half,it was 44.53％.CONCLUSION It suggests that the storage time of P.puberulum should be no less than one year.

Piper puberulumvolatile oilβ-caryophylleneGC-MSstorage time

谢凤凤、曾锦燕、曾祥燕、蔡毅

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广西中医药大学,广西南宁530001

毛蒟 挥发油 β-石竹烯 GC-MS 贮存期

广西自然科学基金创新研究团队项目广西“2011协同创新中心”-壮瑶药协同创新中心项目壮药毛蒟质量标准研究项目广西壮瑶药重点实验广西重点学科(壮药学)

2011GXNSFF018006桂教科研[2013] 20号MZY2013063桂科基字[2014] 32号桂教科研[2013] 16号

2015

10.3969/j.issn.1001-1528.2015.08.031

中成药
国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCDCSCD北大核心
影响因子：1.217
ISSN：1001-1528
年,卷(期)：2015.37(8)
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