摘要
目的 建立UPLC法同时测定护肝片中五味子醇甲、戈米辛J、五味子醇乙、当归酰戈米辛H、当归酰戈米辛Q、五味子酚、五味子甲素、五味子丙素的含量.方法 分析采用WELCH Ultimate©-C18色谱柱(100 mm×2.1 mm,1.8 μm);流动相乙腈-0.1%甲酸,梯度洗脱;体积流量 0.3 mL/min;柱温 40℃;检测波长267 nm.结果 8 种五味子木脂素在各自范围内线性关系良好(R2≥0.998 3),平均加样回收率 96.47%～104.96%,RSD 0.62%～1.60%.结论 该方法简便、快速、准确,可用于护肝片的质量控制.
Abstract
AIM To establish a UPLC method for the simultaneous content determination of schisandrol A,gomisin J,schisandrol B,angeloylgomisin H,angeloylgomisin Q,schisanhenol,deoxyschizandrin and schisandrin C in Waigan Fenghan Granules.METHODS The analysis was performed on a 40℃thermostatic WELCH Ultimate©-C18 column(100 mm×2.1 mm,1.8 μm),with the mobile phase comprising of acetonitrile-0.1%formic acid flowing at 0.3 mL/min in a gradient elution manner,and the detection wavelength was set at 267 nm.RESULTS Eight Schisandra lignans showed good linear relationships within their own ranges(R2≥0.998 3),whose average recoveries were 96.47%-104.96%with the RSDs of 0.62%-1.60%.CONCLUSION This simple,rapid and accurate method can be used for the quality control of Hugan Tablets.
维普
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