首页|交趾黄檀新黄酮类成分及其抗H9c2心肌细胞缺氧/复氧损伤活性研究

交趾黄檀新黄酮类成分及其抗H9c2心肌细胞缺氧/复氧损伤活性研究

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目的 研究交趾黄檀Dalbergia cochinchinensis Pierre ex Laness的新黄酮类成分及其抗H9c2 心肌细胞缺氧/复氧损伤活性.方法 交趾黄檀 70%乙醇提取物采用硅胶、Sephadex LH-20、反相制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构.采用CCK-8法检测其对H9c2 心肌细胞的活性及对H9c2 细胞缺氧/复氧损伤的保护作用,并分析其构效关系.结果 从中分离得到 12 个化合物,分别鉴定为阔叶黄檀酚(1)、5-O-methyllatifolin(2)、mimosifoliol(3)、5-O-methydalbergiphenol(4)、dalbergiphenol(5)、cearoin(6)、2,4-dihydroxy-5-methoxy-benzophenone(7)、2-hydroxy-4,5-dimethoxybenzophenone(8)、melannoin(9)、2,2′,5-trihydroxy-4-methoxybenzophenone(10)、黄檀素(11)、4-甲氧基黄檀醌(12).黄檀酚及黄檀内酯类化合物对H9c2 细胞毒性较小,黄檀酚类化合物抗H9c2 心肌细胞缺氧/复氧损伤活性较强.结论 化合物 8 为新天然产物,化合物 4、9 为首次从该植物中分离得到.黄檀酚类化合物可能是抗H9c2 细胞缺氧/复氧损伤的主要新黄酮类成分.
Neoflavonoids from Dalbergia cochinchinensis and their anti-hypoxia/reoxygenation injury activities on H9c2 myocardial cells
AIM To study the neoflavonoids from Dalbergia cochinchinensis Pierre ex Laness and their anti-hypoxia/reoxygenation injury activities on H9c2 myocardial cells.METHODS The 70%ethanol extract from D.cochinchinensis was isolated and purified by silica gel,Sephadex LH-20 and reverse-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The CCK-8 method was used to detect their activities on H9c2 cells and protective effects on hypoxia-reoxygenation injury of H9c2 cells,and their structure-activity relationship was analyzed.RESULTS Twelve compounds were isolated and identified as latifolin(1),5-O-methyllatifolin(2),mimosifoliol(3),5-O-methydalbergiphenol(4),dalbergiphenol(5),cearoin(6),2,4-dihydroxy-5-methoxy-benzophenone(7),2-hydroxy-4,5-dimethoxybenzophenone(8),melannoin(9),2,2′,5-trihydroxy-4-methoxybenzophenone(10),dalbergin(11),4-methoxydalbergione(12).The dalbergiphenols and dalbergins had little toxicity to H9c2 cells,and dalbergiphenols had strong activity against hypoxia-reoxygenation injury of H9c2 cells.CONCLUSION Compound 8 is a new natural product.Compounds 4,9 are isolated from this plant for the first time.Dalbergiphenols may be the main neoflavonoids against hypoxia-reoxygenation injury of H9c2 cells.

Dalbergia cochinchinensis Pierre ex Lanessneoflavonoidsisolation and identificationH9c2 myocardial cellshypoxia/reoxygenation injurystructure-activity relationship

任佳慧、郑启万、孟晓伟、庞妍、陈兰英、刘荣华

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江西中医药大学药学院, 江西 南昌 330004

江西中医药大学中药固体制剂制造技术国家工程研究中心, 江西 南昌 330006

交趾黄檀 新黄酮 分离鉴定 H9c2心肌细胞 缺氧/复氧损伤 构效关系

国家重点研发计划项目国家自然科学基金江西省自然科学基金江西省中医药管理局科技计划项目江西省自然科学基金重点项目

2018YFC17061028216073220202BABL2160722019A02520224ACB206043

2024

10.3969/j.issn.1001-1528.2024.01.022

中成药
国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCD北大核心
影响因子：1.217
ISSN：1001-1528
年,卷(期)：2024.46(1)
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