首页|窄孢灵芝化学成分及其α-葡萄糖苷酶抑制活性研究

窄孢灵芝化学成分及其α-葡萄糖苷酶抑制活性研究

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目的 研究窄孢灵芝Ganoderma angustisporum J.H.Xing,B.K.Cui&Y.C.Dai的化学成分及其α-葡萄糖苷酶抑制活性.方法 窄孢灵芝乙酸乙酯提取物采用硅胶、ODS、TLC、HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构.采用pNPG法评价其α-葡萄糖苷酶抑制活性.结果 从中分离得到 7 个化合物,分别鉴定为N-acetyl-L-phenylalanine ethyl ester(1)、N-acetyl-L-phenylalanine methyl ester(2)、4-hydroxy-17R-methylincisterol(3)、6,8-di-O-methylaverufin(4)、aversin(5)、methyl 2-(4-hydroxyphenyl)acetate(6)、5-甲苯-1,3-二醇(7).化合物1～2、4～7 的α-葡萄糖苷酶抑制活性IC50值分别为(33.80±0.47)、(45.45±7.95)、(48.80±5.86)、(39.48±2.82)、(41.47±6.68)、(55.38±10.12)μmol/L,其中化合物 1 的抑制活性较强.结论 化合物 1 为新天然产物,化合物 2～7 为首次从灵芝属中分离得到.化合物 1～2、4～7 具有α-葡萄糖苷酶抑制活性.
Chemical constituents from Ganoderma angustisporum and their α-glucosidase inhibitory activities
AIM To study the chemical constituents from Ganoderma angustisporum J.H.Xing,B.K.Cui&Y.C.Dai and their α-glucosidase inhibitory activities.METHODS The ethyl acetate extract from G.angustisporum was isolated and purified by silica gel,ODS,TLC and HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.pNPG method was used to evaluate their α-glucosidase inhibitory activities.RESULTS Seven compounds were isolated and identified as N-acetyl-L-phenylalanine ethyl ester(1),N-acetyl-L-phenylalanine methyl ester(2),4-hydroxy-17R-methylincisterol(3),6,8-di-O-methylaverufin(4),aversin(5),methyl 2-(4-hydroxyphenyl)aceate(6),5-toluene-1,3-diol(7).Compounds 1-2,4-7 showed inhibitory activities of α-glucosidase with IC50 values being(33.80±0.47),(45.45±7.95),(48.80±5.86),(39.48±2.82),(41.47±6.68),(55.38±10.12)μmol/L,and compound 1 showed good inhibitory activity.CONCLUSION Compound 1 is a new natural product.Compounds 2-7 are isolated from genus Ganoderma for the first time.Compounds 1-2,4-7 have α-glucosidase inhibitory activities.

Ganoderma angustisporum J.H.Xing,B.K.Cui&Y.C.Daichemical constituentsisolation and identificationα-glucosidase inhibitory activities

霍雅琴、王雨曦、魏玉莲、张怡轩、袁海生

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沈阳药科大学生命科学与生物制药学院, 辽宁 沈阳 110016

中国科学院沈阳应用生态研究所,中国科学院森林生态与管理重点实验室, 辽宁 沈阳 110016

窄孢灵芝 化学成分 分离鉴定 α-葡萄糖苷酶抑制活性

国家自然科学基金青年项目中国博士后科学基金面上项目辽宁省应用基础研究计划项目沈阳市科技计划项目

321000132022M7132962022JH2/10130018222-322-3-07

2024

10.3969/j.issn.1001-1528.2024.01.023

中成药
国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCD北大核心
影响因子：1.217
ISSN：1001-1528
年,卷(期)：2024.46(1)
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