摘要
目的 建立UPLC-MS/MS法同时测定养血软坚胶囊中芹糖甘草苷、芍药内酯苷、獐牙菜苦苷、没食子酸甲酯、苯甲酰芍药苷、獐牙菜苷、6′-O-β-D-葡萄糖基龙胆苦苷、异甘草素、马钱苷酸、甘草素、没食子酸、芍药苷、氧化芍药苷、龙胆苦苷、甘草酸、异甘草苷、甘草苷的含量.方法 分析采用Waters BEH C18 色谱柱(2.1 mm×100 mm,1.7 μm);流动相 2 mmol/L乙酸铵(含 0.1%甲酸)-乙腈,梯度洗脱;体积流量 0.3 mL/min;柱温 40℃;电喷雾离子源;负离子扫描;多反应监测模式.结果 17 种成分在各自范围内线性关系良好(r>0.999 6),平均加样回收率91.33%～104.03%,RSD 1.58%～3.50%.结论 该方法快速、准确、稳定,可用于养血软坚胶囊的质量控制.
Abstract
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of liquiritin apioside,alibiflorin,swertiamarin,methyl gallate,benzoylpaeoniflorin,sweroside,6′-O-β-D-glucosylgentiopicroside,isoliquiritigenin,loganic acid,liquiritigenin,gallic acid,paeoniflorin,oxypaeoniflorin,gentiopicroside,glycyrrhizic acid,isoliquiritoside and liquiritin in Yangxue Ruanjian Capsules.METHODS The analysis was performed on a 40℃thermostatic Waters BEH C18column(2.1 mm×100 mm,1.7 μm),with the mobile phase comprising of 2 mmol/L ammonium acetate(containing 0.1%formic acid)-acetonitrile flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.RESULTS Seventeen constituents showed good linear relationships within their own ranges(r>0.999 6),whose average recoveries were 91.33%-104.03%with the RSDs of 1.58%-3.50%.CONCLUSION This rapid,accurate and stable method can be used for the quality control of Yangxue Ruanjian Capsules.
基金项目
国家自然科学基金面上项目(82074466)
国家自然科学基金面上项目(82174403)
上海市慢性筋骨病临床医学研究中心项目(20MC1920600)
上海市临床重点专科"中医骨伤科"项目(shslczdzk03901)
全国中医学术流派传承工作室建设项目(第二轮)()
上海高水平地方高校"慢性筋骨病损研究与转化"创新团队项目(沪教委人[2022]3号)
海派中医流派传承延伸计划(2022-75)
维普
万方数据