摘要
目的 鉴定臭常山内生真菌G-(JK)-2,并对次级代谢产物及其α-糖苷酶抑制活性进行研究.方法 通过ITS序列建立系统进化树,鉴定内生真菌G-(JK)-2;对其 45d大米固体培养基发酵物进行甲醇提取、乙酸乙酯萃取,硅胶、Sephadex LH-20 凝胶和半制备HPLC分离纯化后进行结构鉴定,采用PNPG法评价α-糖苷酶抑制活性.结果 鉴定臭常山内生真菌G-(JK)-2 为镰刀属内生真菌Fusarium nematophilum;分离得到 13 个化合物,分别鉴定为p-hydroxybenzaldehyde(G1)、4-hydroxyacetophenone(G2)、anhydromevalonolactone(G3)、flazine(G4)、salicylic acid(G5)、p-hydroxybenzoic acid(G6)、di-(2-ethylhexyl)-phthalate(G7)、terephthalic acid bis(2-ethyl-hexyl)ester(G8)、thymine(G9)、uridine(G10)、adenosine(G11)、2′-deoxyuridine(G12)、nicotinic acid(G13).各化合物对α-葡萄糖苷酶抑制作用依次为G4>G11>G10>G13>G12.结论 所有化合物均首次从臭常山的内生真菌中分离得到,G10、G11、G13 首次从镰刀属内生真菌中分离得到,G4 和G11 有较弱的α-葡萄糖苷酶抑制作用.
Abstract
AIM To identify the endophytic fungus G-(JK)-2 from Orixa japonica Thunb.and to study its secondary metabolites and their α-glucosidase inhibitory activities.METHODS Through the ITS sequence,the evolutionary tree that identifies the endophytic fungus G-(JK)-2 was established.Then 45 days rice solid medium of endophytic fungus G-(JK)-2 was extracted by methanol,and then by ethyl acetate.The ethyl acetate extract was separated and purified by silica gel chromatography,Sephadex LH-20,and semi-preparative HPLC.The structures of obtained compounds were identified by physicochemical properties and spectral data.Their α-glucosidase inhibitory activities were evaluated by PNPG method.RESULTS The endophytic fungus G-(JK)-2 from O.japonica was identified as Fusarium nematophilum.Thirteen compounds were isolated and identified as p-hydroxybenzaldehyde(G1),4-hydroxyacetophenone(G2),anhydromevalonolactone(G3),flazine(G4),salicylic acid(G5),p-hydroxybenzoic acid(G6),di-(2-ethylhexyl)-phthalate(G7),terephthalic acid bis(2-ethyl-hexyl)ester(G8),thymine(G9),uridine(G10),adenosine(G11),2′-deoxyuridine(G12),nicotinic acid(G13).The inhibitory effect of each compound on α-glucosidase was in sequence of G4>G11>G10>G13>G12.CONCLUSION All compounds are first isolated from the endophytic fungi of the O.japonica,and G10,G11,G13 are first isolated from the endophytic fungi of Fusarium.G4 and G11 have mild inhibition to α-glucosidase.
基金项目
中央引导地方科技发展项目(黔科中引地[2021]4046号)
特殊医学用途配方食品技术研发创新平台建设项目(黔科合服企[2019]4001号)
维普
万方数据