首页|基于UPLC-Q-TOF-MS的龙牡清心合剂成分鉴定及其治疗注意缺陷多动障碍的物质基础研究

基于UPLC-Q-TOF-MS的龙牡清心合剂成分鉴定及其治疗注意缺陷多动障碍的物质基础研究

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目的 采用UPLC-Q-TOF-MS技术鉴定龙牡清心合剂化学成分,探究其治疗注意缺陷多动障碍的物质基础.方法 采用Waters CORTECS©UPLC© T3 色谱柱在正、负离子模式下对样品进行质谱检测,以Peakview 1.2 软件进行数据分析,与Natural Products HR-MS/MS Spectral Library 1.0 数据库匹配,结合文献报道进行成分鉴定,结合鉴定结果分析龙牡清心合剂治疗注意缺陷多动障碍的物质基础.结果 共鉴定出 40 种化学成分,包括黄酮类 11种、单萜苷类 6 种、三萜皂苷类 4 种、酚酸类 3 种、生物碱类 6 种等,主要来源于黄芪、白芍、黄芩、甘草、钩藤等.黄芩苷、黄芩素、芒柄花黄素、黄芪甲苷、钩藤碱可能是龙牡清心合剂治疗注意缺陷多动障碍的物质基础.结论 UPLC-Q-TOF-MS可快速对龙牡清心合剂中的化学成分进行鉴定,黄酮类、三萜皂苷类、生物碱类成分可能是龙牡清心合剂治疗注意缺陷多动障碍的物质基础,可为该方的物质基础研究、质量标准建立、拆方药理学研究提供依据.
Identification of chemical components of Longmu Qingxin Mixture by UPLC-Q-TOF-MS and research on its material basis for attention deficit hyperactivity disorder
AIM To identify the chemical components of Longmu Qingxin Mixture by UPLC-Q-TOF-MS and study its material basis for the treatment of attention deficit hyperactivity disorder.METHODS The sample was detected by mass spectrometry in positive and negative ion mode on a Waters CORTECS© UPLC© T3 chromatographic column.The data were analyzed with Peakview 1.2 software and matched with the Natural Products HR-MS/MS Spectral Library 1.0 database,and the components were identified in combination with literature reports.The material basis of Longmu Qingxin Mixture for the treatment of attention deficit hyperactivity disorder was analysed according to the identified components.RESULTS Forty chemical components were identified,including 11 flavonoids,6 monoterpene glycosides,4 triterpene saponins,3 phenolic acids,6 alkaloids etc.,which mainly derived from Radix Astragali,Radix Paeoniae Alba,Radix Scutellariae,licorice root,Ramulus Uncariae cum,etc.,baicalein,formononetin,astragaloside Ⅳ and rhynchophylline may be the material basis for the therapeutic effect of Longmu Qingxin Mixture.CONCLUSION UPLC-Q-TOF-MS can quickly identify the chemical components of Longmu Qingxin Mixture.Flavonoids,triterpene saponins and alkaloids may be the material basis for Longmu Qingxin Mixture for the treatment of attention deficit hyperactivity disorder,which can provide the basis for its material basis research,quality standard establishment and pharmacological study of the dismantled formula.

Longmu Qingxin Mixtureattention deficit hyperactivity disorderUPLC-Q-TOF-MSfingerprintchemical compositionpharmacological action

李雪军、姜之炎、肖臻、陈秀峰、王淑敏、张奕星、蒲文焱

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上海中医药大学附属龙华医院儿科, 上海 200032

龙牡清心合剂 注意缺陷多动障碍 UPLC-Q-TOF-MS 指纹图谱 化学成分 药理作用

上海市进一步加快中医药传承创新发展三年行动计划(2021年-2023年)项目上海市临床重点专科建设项目龙华医院科技创新项目

ZY2021-2023-0206-01shslczdzk04102KY22059

2024

10.3969/j.issn.1001-1528.2024.02.023

中成药
国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCD北大核心
影响因子：1.217
ISSN：1001-1528
年,卷(期)：2024.46(2)
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