首页|基于液质联用技术比较郁香忍冬花蕾与金银花化学成分

基于液质联用技术比较郁香忍冬花蕾与金银花化学成分

Comparison of chemical components of Lonicera fragrantissima and Lonicera japonica based on LC-MS

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目的 比较郁香忍冬花蕾与金银花的成分差异,评估郁香忍冬花蕾药用价值,为其开发利用提供参考.方法 以 70%甲醇为提取溶剂,以UPLC-TOF-MS分析成分种类,以HPLC-QQQ-MS测定成分含量,经多元统计分析确定成分差异.结果 在郁香忍冬花蕾与金银花中共鉴定出 52 种成分,20 种定量成分含量差异显著,金银花有 4 种差异成分,郁香忍冬花蕾的异绿原酸C、阿魏酸、木犀草苷、芦丁含量是金银花的 2 倍以上,马钱酸、马钱苷含量分别是金银花的11.96、37.23 倍.马钱苷、异绿原酸A、马钱酸、芦丁及断马钱酸是区分郁香忍冬花蕾及金银花的关键差异成分.结论 郁香忍冬花蕾与金银花成分种类相近,但含量差异明显,郁香忍冬花蕾具有重要药用价值,可进一步开发利用.
AIM To compare the components difference between Lonicera fragrantissima Lindl.et Paxt.(LFL)and Lonicerae japonicae Flos(LJF),and to evaluate the medicinal value of LFL,so as to provide reference for the development and utilization of LFL and LJF.METHODS With 70%methanol as extraction solvent,the components were analyzed by UPLC-TOF-MS,and the contents of 20 components were determined by HPLC-QQQ-MS.The components difference was determined by multivariate statistical analysis.RESULTS A total of 52 components were identified in the buds of LFL and LJF.There were 4 different components in LJF,and the contents of 20 quantitative components were significantly different.The contents of isochlorogenic acid C,ferulic acid,luteolin and rutin in the buds of LFL were more than 2 times that of LJF,and the contents of marchanic acid and marchanin were 11.96 times and 37.23 times that of LJF respectively.Maganin,isochlorogenic acid A,maganic acid,rutin and dicomachanic acid are the key differentiating components of LFL and LJF.CONCLUSION The buds of LFL and LJF have similar species,but the content difference is obvious.The buds of LFL have important medicinal value,which need further development and utilization.

Lonicera fragrantissima Lindl.et Paxt.Lonicerae japonicae Flos.LC-MSmultivariate statistical analysis

金莹、熊乐文、蒲高斌、张芳、李佳、张龙霏、张永清

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山东中医药大学,济南 长清 250300

郁香忍冬 金银花 液质联用 多元统计分析

国家重点研发计划国家自然科学基金山东省重大科技创新工程项目山东省重点研发计划

2017YFC1701503818729632019JZZY0110202016CYJS08A01

2024

中成药
国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCD北大核心
影响因子:1.217
ISSN:1001-1528
年,卷(期):2024.46(3)
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