Simultaneous content determination of ten constituents in Baihe Zhimu Decoction by UPLC-MS/MS
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of sarsasapogenin,regaloside C,timosaponin A Ⅲ,mangiferin,neomangiferin,regaloside A,regaloside B,timosaponin BⅢ,isomangiferin and timosaponin BⅡ in Baihe Zhimu Decoction.METHODS The analysis was performed on a 30℃thermostatic Agilent Poroshell 120 EC-C18 column(3.0 mm×150 mm,2.7 μm),with the mobile phase comprising of water(containing 0.1%formic acid)-acetonitrile(containing 0.1%formic acid)flowing at 0.5 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Ten constituents showed good linear relationships within their own ranges(r>0.998 1),whose average recoveries were 95.65%-106.5%with the RSDs of 1.2%-5.5%.CONCLUSION This accurate,sensitive and rapid method can be used for the quality control of Baihe Zhimu Decoction.
国家科技期刊平台
NSTL
万方数据
维普
