一测多评法同时测定心脑康胶囊中9种成分
Simultaneous determination of nine constituents in Xinnaokang Capsules by QAMS
刘康康 1靳婉君 2浩文婷 1刘富强 2赖晶 2蔺瑞丽 2倪琳2
作者信息
- 1. 甘肃中医药大学药学院, 甘肃 兰州 730000
- 2. 甘肃省药品检验研究院, 国家中药材及饮片质量控制重点实验室, 甘肃省中藏药检验检测技术工程实验室, 甘肃 兰州 730030
- 折叠
摘要
目的 建立一测多评法同时测定心脑康胶囊中丹参素钠、丹酚酸B、芍药苷、阿魏酸、羟基红花黄色素A、2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、斯皮诺素、甘草苷、葛根素的含量.方法 分析采用SVEA C18 色谱柱(250 mm×4.6 mm,5 μm);流动相乙腈-0.2%甲酸,梯度洗脱;体积流量 0.5 mL/min;柱温 30℃;检测波长 280、403 nm.以葛根素为内标,计算其他 8 种成分的相对校正因子,计算其含量.结果 9 种成分在各自范围内线性关系良好(r≥0.999 4),平均加样回收率 99.83%～101.18%,RSD 1.04%～1.91%.一测多评法所得结果与外标法接近.结论 该方法准确稳定,重复性好,可用于心脑康胶囊的质量控制.
Abstract
AIM To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of sodium danshensu,salvianolic acid B,paeoniflorin,ferulic acid,hydroxysafflor yellow A,2,3,5,4′-tetrahydroxy stilbene-2-Ο-β-D-glucoside,spinosin,liquiritin and puerarin in Xinnaokang Capsules.METHODS The analysis was performed on a 30℃thermostatic SVEA C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2%formic acid flowing at 0.5 mL/min in a gradient elution manner,and the detection wavelengths were set at 280,403 nm.Puerarin was used as an internal standard to calculate the relative correction factors of the other eight constituents,after which the content determination was made.RESULTS Nine constituents showed good linear relationships within their own ranges(r≥0.999 4),whose average recoveries were 99.83%-101.18%with the RSDs of 1.04%-1.91%.The result obtained by QAMS approximated those obtained by external standard method.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of Xinnaokang Capsules.
关键词
心脑康胶囊/化学成分/含量测定/一测多评Key words
Xinnaokang Capsules/chemical constituents/content determination/quantitative analysis of multi-components by single-marker(QAMS)引用本文复制引用
基金项目
甘肃省药品科研项目(2022GSMPA-KL12)
甘肃省药品科研项目(2022GSMPA-KL14)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据