Analyses of chemical constituents and constituents absorbed into blood in Zixin Biqiu Granules
AIM To establish a UPLC-Q-TOF-MS/MS method for the analyses of chemical constituents and constituents absorbed into blood in Zixin Biqiu Granules.METHODS The analysis was performed on a 30℃thermostatic Agilent ZORBAX SB-C18 column(1.8 μm,2.1 mm×100 mm),with the mobile phase comprising of 0.2%formic acid-acetonitrile flowing at 0.2 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode,after which constituent identification was employed according to excimer ions,retention time,secondary fragment ions combined with reference substance information,relevant literatures.RESULTS Total 88 constituents were identified,28 of which were validated by comparison of reference substances.Thirty constituents were observable in rat serum,20 of which were prototype constituents,10 of which were metabolites.CONCLUSION This stable and reliable method can lay the foundation for research on pharmacological substance basis and quality control of Zixin Biqiu Granules.
Zixin Biqiu Granuleschemical constituentsconstituents absorbed into bloodUPLC-Q-TOF-MS/MS
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